Arthrocentesis

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Chapter 53

Arthrocentesis

Background

Arthrocentesis, the puncture and aspiration of a joint, is an acknowledged, useful procedure that is easily performed in the emergency department (ED).1 It has been established as both a diagnostic and therapeutic tool for various clinical situations. When performed properly, the procedure offers a wealth of clinical information and is associated with few complications. In the ED it is difficult to make an accurate assessment of an acutely painful, hot, and swollen joint without performing arthrocentesis.

Indications and Contraindications

The indications for arthrocentesis are listed in Review Box 53-1.

Infection in the tissues overlying the site to be punctured is generally considered an absolute contraindication to arthrocentesis. However, inflammation with warmth, swelling, and tenderness may overlie an acutely arthritic joint, and this condition may mimic a soft tissue infection. Once convinced that cellulitis does not exist, the clinician should not hesitate to obtain the necessary diagnostic joint fluid. Known bacteremia is a theoretical relative contraindication because infection can spread to the joint; however, this complication should be weighed against the useful information and culture results gained by fluid analysis. A unique indication—or a serendipitous finding during arthrocentesis—is identification of fat globules in joint blood (lipohemarthrosis), which signifies an occult fracture.

Bleeding diatheses are rarely a relative contraindication, and arthrocentesis to relieve a tense hemarthrosis in bleeding disorders such as hemophilia is an accepted practice after infusion of the appropriate clotting factors. There are few data regarding the safety or dangers of arthrocentesis in patients taking anticoagulants or platelet inhibitors. Studies have demonstrated that the risk for iatrogenic hemarthrosis in patients treated with oral anticoagulants is extremely low, even in those who have international normalized ratios as high as 4.5.2 One prospective trial of 32 patients taking warfarin found no complications after athrocentesis.3 Hence, when necessary, arthrocentesis should be performed in patients taking anticoagulants. The value of reversing a coagulopathy with blood components before the procedure is not proved, and clinical judgment should prevail. Prosthetic joints are at high risk for infection, and arthrocentesis should be avoided whenever possible in this situation. However, if an infected prosthesis is suspected, arthrocentesis should be performed.

Articular versus Periarticular Disease

Periarticular conditions such as trauma, tendinitis, bursitis, contusion, cellulitis, or phlebitis may mimic articular disease and suggest the need for arthrocentesis. Therefore, evaluation of acute joint disease requires that the clinician first determine whether the patient’s constellation of signs and symptoms derives from the joint itself or from some other musculoskeletal or periarticular structure. Such a distinction, however, may be difficult, if not impossible to make without analysis of synovial fluid. No specific test or physical finding has high specificity for solving this dilemma; however, some physical findings may prove helpful. A common periarticular structure that can be associated with a joint effusion is a Baker cyst (popliteal cyst).

If the swelling is secondary to joint effusion or inflammation, the entire articular capsule will be inflamed and distended and fluid can often be palpated within the joint. In the knee, this condition must be differentiated from effusion into the prepatellar bursa, where swelling distends the bursa that lies mainly over the lower portion of the patella, between it and the skin. Effusion into the joint occurs posterior to the patella, whereas bursal swelling occurs anterior to it (Fig. 53-1). When considerable articular effusion of the knee is present, the capsule of the joint is distended and an inverted U-shaped swelling of the joint develops. This characteristic shape occurs because the dense patellar ligament prevents distention of the capsule along its inferior border. Also, with the knee extended a large effusion causes the patella to “float” or lift away from the femoral condyles. Complete extension and flexion are often impossible because of the joint tension produced by the effusion.

Joint effusion causes limited movement of the joint in all directions, with active and passive motion producing pain. The pain arising from a pathologic condition involving a joint may be diffuse or clearly localized to the joint, or it may radiate. Hip pain, for example, frequently radiates into the groin or down the front of the thigh into the knee. Shoulder joint pain commonly radiates into the elbow or the neck. Therefore, complete examination of contiguous structures is essential for adequate diagnosis.

In contrast, pain from a periarticular process is often more localized, and tenderness can be elicited only with certain specific movements or at specific points around the joint. In periarticular inflammation, one can often passively lead a joint through a range of motion with minimal discomfort, yet pain is significant when the patient attempts active motion. Crepitus may be elicited with tendinitis, or the pain may be traced along the course of a specific tendon.

Septic Arthritis

Acute monarticular arthritis is a common problem in emergency medicine. Although acute monarticular arthritis has many causes, septic arthritis is the one requiring most urgent diagnosis and treatment. Infectious arthritis is still relatively frequent, and suspicion of a septic process in the joint is the first step in appropriate management; confirmation requires arthrocentesis and culture of synovial fluid. In the ED, synovial fluid analysis is the diagnostic test most heavily relied on in making the diagnosis of an acute intraarticular infection. Culture remains the most definitive study, although it is not 100% sensitive.4 Gram stain may be helpful, but a negative Gram stain does not exclude the presence of a joint infection because not all infected joints have a positive Gram stain. Therapeutic arthrocentesis might be need to be repeated when treating a septic joint. Such therapy is usually performed on an inpatient basis.5

Infection of a joint occurs by one of several mechanisms: hematogenous spread (bacteremia, infective endocarditis, intravenous drug use), spread from a contiguous source of infection, direct implantation, postoperative contamination, or trauma.4 Septic arthritis is typically monarticular with a swollen, erythematous, and painful joint. The noninfectious differential diagnosis includes crystal-induced arthritis, fracture, hemarthrosis, foreign body, osteoarthritis, ischemic necrosis, and monarticular rheumatoid arthritis. In addition, osteomyelitis may mimic septic arthritis because of the close proximity of the infected metaphysis to the joint space.6 In many instances an acutely inflamed joint from gout or other arthritides simply cannot be distinguished from infection clinically. Nonetheless, early diagnosis is essential to prevent complications such as impairment of growth, articular destruction with ankylosis, osteomyelitis, and soft tissue extension.7

Because an acutely swollen joint may be indicative of a number of disease entities, a thorough history and physical examination are the cornerstones of evaluation, followed by arthrocentesis (Fig. 53-2). Laboratory findings can be useful in making a diagnosis, as can response to therapy (e.g., the response to empirical antibiotics in gonococcal arthritis is often the only criterion for diagnosis because the organism is difficult to culture). Blood cultures may be positive since joint infections may be due to hematogenous spread. Patients with malignancy (especially leukemia) or those who are immunosuppressed or otherwise debilitated are at particular risk for a septic cause. Infectious arthritis should be considered primarily in these patients, as well as in those with preexisting joint diseases such as rheumatoid arthritis. In general, a swollen joint is not usually injected with corticosteroids until the possibility of infection has been eliminated.8

Neisseria gonorrhoeae, Staphylococcus (including methicillin resistant), and Streptococcus are the most frequently identified etiologic agents. N. gonorrhoeae is the most common organism causing septic arthritis in adolescents and young adults. Patients older than 40 years and those with other medical illnesses are more likely to have Staphylococcus joint infections. In children, Staphylococcus, Streptococcus, and Escherichia coli predominate. Haemophilus influenzae was a common cause of pediatric septic arthritis in the past, but widespread use of the conjugate vaccine has reduced H. influenzae infection rates to nearly zero.810 In neonates, staphylococci, Enterobacteriaceae, group B streptococci, and N. gonorrhoeae are the most likely organisms. Staphylococcal or pseudomonal infections commonly develop in injection drug abusers. Salmonella arthritis is more prevalent in patients with sickle cell disease than in the general population; however, more common organisms still predominate. Prosthetic joints or postoperative infections have high rates of Staphylococcus aureus, Streptococcus epidermidis, Enterobacteriaceae, and Pseudomonas infection.11

The prevalence of community-acquired methicillin-resistant S. aureus (CA-MRSA) mandates special attention. Epidemiologic data on the incidence of CA-MRSA septic arthritis are sparse; however, one recent study noted that 50% of synovial fluid cultures in suspected septic arthritis ultimately grew MRSA.12 It would be prudent to consider empirical therapy for MRSA in those suspected of having septic arthritis until the results of culture become available. MRSA-infected joints can be multiple, progress rapidly, and be very destructive of joint tissue and adjacent bone.

Although precise incidence data for nongonococcal septic arthritis have not been established, predisposing factors have been described and include age 80 years or older, diabetes mellitus, rheumatoid arthritis, hip or knee prosthesis, joint surgery, and skin infection.13 The simultaneous occurrence of gout and septic arthritis is possible, and one should not allow the establishment of a diagnosis of crystal-induced disease to stop a thorough search for infection.14

Because N. gonorrhoeae is the most common organism causing septic arthritis, gonococcal arthritis deserves special mention. Disseminated gonococcal infection occurs in 0.5% to 3% of cases of mucosal infection. Gonococcal septic arthritis is more common in women, especially during pregnancy or after menstruation, because women with sexually transmitted gonorrhea infections are more likely to be asymptomatic. The time needed for local infection to disseminate can vary from several days to weeks. Patients will often experience systemic symptoms, including fevers, chills, and malaise, as well as migratory polyarthralgia. Gonococcal tenosynovitis without joint involvement occurs in two thirds of patients. Dermatitis is also present in two thirds of patients (Fig. 53-3). The most common rash consists of scattered painless, nonpruritic 0.5- to 0.75-cm macules or papules with necrotic or pustular centers distributed on the extremities and trunk. Eventually, the infection settles into one or two large joints to yield a purulent arthritis.9,15 Overt urethritis and vaginitis may be absent or overlooked because of concentration solely on the obvious joint pathology. Hence, it is important to realize that disseminated gonococcal infections can be associated with surprisingly minimal or even absent signs and symptoms of a genital infection source. Some joints may become inoculated through hematogenous spread from anal and oral sites of infection. Even though N. gonorrhoeae–infected joint fluid is usually “septic” in character, the yield of positive synovial fluid cultures has ranged from 25% to 50%. Blood cultures appear to be less helpful since they are positive in only 20% to 30% of cases. Because blood and joint fluid culture has a low yield, it would be prudent to culture all possible sites of gonococcal infection, including anal and pharyngeal sites. The organism can often be identified in asymptomatic genitourinary sites,9 with cultures from the primary mucosal site being positive in up to 80% of infected patients.15

A positive Gram stain is immediately diagnostic of septic arthritis. However, Gram stains are positive in only 71% of gram-positive infections, 40% to 50% of gram-negative infections, and 25% of gonococcal infections.16,17 An elevated synovial white blood cell (WBC) count and a reduction in synovial fluid glucose may give confirmatory data. However, the synovial fluid WBC count in gonococcal arthritis is often between 10,000 and 20,000 cells/mm.3,4 Although mild leukocytosis and an elevated erythrocyte sedimentation rate may occur, normal laboratory values do not exclude infection.16,18

Hemarthrosis

Isolated nontraumatic hemarthrosis may occasionally be seen by the emergency clinician. An inflammatory reaction may follow intracapsular bleeding, and the proliferative reaction and the hyperplastic synovium formed might predispose patients to recurrent hemorrhage in that joint, especially those with bleeding diatheses. The knee is the most commonly affected joint, followed by the ankle, elbow, shoulder, and hip.1

The most common cause of intraarticular hemorrhage in the setting of no or minor trauma is a hereditary clotting factor deficiency such as hemophilia. Hemarthrosis is an infrequent complication of oral anticoagulant therapy but might occur even with prothrombin times within the normal range.19 Cessation of anticoagulant therapy in these patients must be weighed against the risk for adverse clot formation (e.g., acute cerebrovascular accident). Chronic arthritis does not appear to be a long-term complication in patients with intraarticular bleeding from oral anticoagulant therapy. Hemarthrosis may also be a complication of sickle cell anemia, pseudogout, amyloidosis, pigmented villonodular synovitis, synovial hemangioma, rheumatoid arthritis, and infection.18,20

Management of acute hemarthrosis depends on the cause. Hemarthrosis associated with oral anticoagulant therapy improves only after use of the oral anticoagulant is discontinued and the prothrombin time returns to normal. Hemarthrosis after trauma is a frequent occurrence. It is most common in the knee and often denotes significant internal damage. A massively swollen knee after trauma is frequently seen with knee dislocation (occasionally with spontaneous relocation) and a tear of the anterior cruciate ligament. Intraarticular fractures can cause a significant hemarthrosis.

Distension of the joint by effusion or hemorrhage causes considerable pain and disability. If the fluid is not removed, it is partially absorbed, but part of it may undergo organization and result in the formation of adhesions or bands in the joint. This is one argument for drainage of the joint.2 Some believe that in an otherwise healthy joint that is subjected to a single traumatic event, even a relatively large hemarthrosis will be spontaneously reabsorbed without significant sequelae and therefore presents no pressing need for drainage. Unfortunately, no literature exists to guide the best approach; hence, there is no universal standard of care regarding the need or lack thereof of draining blood from a traumatic joint.

Nonetheless, a large, tense, traumatic effusion is quite painful, and its presence precludes proper evaluation of an injured joint. Therapeutic arthrocentesis to drain a symptomatic traumatic effusion is a common and well-accepted practice.2,19,21,22 The source of blood after trauma is frequently a tear in a ligamentous structure, capsule, or synovium or a fracture. Cruciate (especially anterior) ligament injury is the most common cause of significant hemarthrosis after trauma to the knee.20 A joint effusion that develops 1 to 5 days after trauma may be secondary to a slow hemorrhage or reinjury, but the swelling is often caused by a nonhemorrhagic irritative synovial effusion.

Occasionally, one will diagnose an occult fracture by the presence of lipohemarthrosis, or fat globules in the arthrocentesis specimen (Fig. 53-4). This may be appreciated when the bloody effusion is placed in a clear container (e.g., emesis basin) and held to a light. If the history of trauma is vague, arthrocentesis may be required to differentiate hemorrhage from other causes of joint effusion. An occult tibial plateau fracture is an example in which evaluating for lipohemarthrosis may be of particular value. Following therapeutic arthrocentesis for a hemarthrosis, it may be desirable to inject 2 to 15 mL, depending on joint size, of a long-acting local anesthetic (see Chapter 29) into the joint to facilitate examination or provide temporary relief of the symptoms.

Intraarticular Corticosteroid Injections

In 1951 Hollander and coworkers23 first demonstrated that intraarticular corticosteroid injections are useful for relief of symptoms in patients with severe rheumatoid arthritis. The use of steroids has proved to be a dependable method for providing rapid relief of pain and swelling of inflamed joints, although it is strictly local, usually temporary, and rarely curative.2,24,25 It is easily performed in the emergency setting. Acute gout responds well to joint injection, and this may be preferable in patients who cannot tolerate indomethacin or colchicine.

Corticosteroid injections are most helpful when only a small number of joints are actively inflamed. The most frequently used corticosteroids for intraarticular injection are shown in Table 53-1.2 Diminution of joint pain, swelling, effusion, and warmth is usually evident within 6 to 12 hours after injection.

TABLE 53-1

Intrasynovial Corticosteroid Preparations*

image

*Listed in approximate descending order of duration of action.

The dose will depend on joint size, capsular distensibility, and degree of inflammation.

From Gray RG, Gottlieb NL. Corticosteroid injections in RA: appraisal of a neglected therapy. J Musculoskelet Med. 1990;7:53. Reproduced by permission.

Though very rare, the most serious complication of this practice is intraarticular infection.2 Therefore, steroids should not be injected into a joint if a joint space infection is suspected. Repeated injections into one joint pose a risk for necrosis of juxtaarticular bone with subsequent joint destruction and instability and suppression of the hypothalamic-pituitary axis from systemic absorption. Other complications include local soft tissue atrophy and calcification, tendon rupture, intraarticular bleeding, and transient nerve palsy.2,25 Transient elevations in blood glucose, as well as erythema, warmth, and diaphoresis of the face and torso, may also occur after intraarticular steroid injections. Acute pain, redness, and swelling 12 to 24 hours after steroid injection can mimic infection, but with this timing it is most likely an inflammatory reaction (steroid flare) to crystal-containing steroid preparation (often methylprednisolone). Deposition of steroid crystals on the synovium might give rise to a transient, self-limited flare-up of synovitis.2,26

It is always important to determine whether local corticosteroid therapy has been used previously, not only to consider the array of clinical conditions associated with steroid use but also because crystalline corticosteroid material can hinder proper interpretation of crystals found in synovial fluid.26

Equipment

The material needed for arthrocentesis includes skin preparation solutions (e.g., chlorhexidine); sterile gloves and drapes (optional in some cases); local anesthetic; syringes for injecting local anesthetic and aspirating joint fluid; a three-way stopcock for draining large amounts of fluid; lavender-, red-, and green-topped blood tubes; and various sizes of needles and intravenous catheters (see Review Box 53-1).

Depending on the size of the effusion to be drained, a 10-, 20-, or 30-mL Luer-Lok syringe can be used. If a large effusion is suspected, a three-way stopcock between the needle and the syringe allows complete drainage with a single joint penetration. Fluid for cell count should be collected in a lavender-topped tube; however, viscosity, protein, and glucose determinations do not require anticoagulants, and fluid should be placed in a red-topped tube. Though still common practice in many institutions, recent evidence suggests that synovial fluid protein and glucose levels are poor differentiators of noninflammatory and inflammatory effusions and are no longer recommended (see “Synovial Fluid Interpretation,” later).2,2729 Immediately examine fresh synovial fluid in its unadulterated form for crystals. Calcium oxalate and lithium heparin anticoagulants have been reported to introduce artifactual crystals into the fluid. Joint fluid to be analyzed for crystals should be collected in a green-topped tube containing sodium heparin. If culturing for N. gonorrhoeae, the fluid should be immediately placed on proper medium and stored in a low-oxygen environment in the ED.

imageULTRASOUND

Arthrocentesisby Christine Butts, MD

Ultrasound offers a significant advantage when evaluating a patient with a complaint of joint pain. Patients with obesity or significant pain limiting physical examination make the diagnosis of joint effusion difficult. Attempting blind aspiration in these patients may cause significant pain in the patient and frustration in the clinician. Ultrasound allows the physician to thoroughly evaluate the joint space for the presence of effusion and to plan the best approach for aspiration. The initial evaluation of the major joints is discussed below, followed by a general approach to aspiration.

Knee

Although effusions of the knee are frequently diagnosed on physical examination alone and aspirated blindly, ultrasound allows the clinician to distinguish effusion from other conditions that may cause generalized swelling (such as bursitis).

A high-frequency transducer (7.5 to 10 mHz) should be used to allow the greatest resolution. Begin with the indicator pointing toward the patient’s head (in longitudinal orientation) over the anterior aspect of the knee and attempt to locate the patella (Fig. 53-US1). The patella can be seen as a brightly echogenic (white) object with posterior shadowing (Fig. 53-US2). Locating the patella is key to distinguishing prepatellar bursitis, which will appear as a dark, fluid-filled collection superficial to the patella, and a joint effusion, which will appear as a dark, fluid-filled collection deep to the patella. Once the patella has been identified, the transducer should be moved medially or laterally to “look under” the patella into the joint space (Fig. 53-US3). Fluid will appear as a dark gray or black collection between the articular surface of the femur and fibula or tibia (Fig. 53-US4). Once this area has been evaluated, the transducer should be moved superiorly to evaluate the suprapatellar bursa, which lies superior to the patella and deep to the quadriceps tendon. The suprapatellar bursa communicates with the joint space and frequently houses a large amount of fluid (Fig. 53-US5).

Shoulder

Either an anterior or posterior approach can be used to evaluate the shoulder. In the anterior approach, the patient should first be placed in a seated position with the elbow adducted and the palm facing up. The high-frequency transducer can then be placed in a transverse orientation over the approximate location of the biceps tendon (Fig. 53-US6). The biceps tendon is an extracapsular extension of the joint and will be seen to distend with fluid when a joint effusion is present. A normal tendon can be seen to lie within the biceps groove of the humerus (Fig. 53-US7). When surrounded by fluid, the tendon will be seen to “float” within an anechoic (black) area (Fig. 53-US8).

To evaluate the joint from the posterior approach, place the patient in a seated position with the affected hand on the opposite shoulder to open the joint space. The transducer can then be placed at the approximate location of the articulation of the humeral head with the glenoid (Fig. 53-US9). In a normal joint, the humerus can be seen to articulate with the glenoid without any intervening fluid (Fig. 53-US10). When an effusion is present, a dark gray or black collection can be seen medial to the glenoid (Fig. 53-US11).

Ankle

The ankle joint is best evaluated in the longitudinal axis with the transducer placed over the space between the posterior edge of the tibia and the talus (Fig. 53-US12). Slightly plantar-flexing the foot will enable the transducer to “fit” in this space. In a normal joint, the distal edge of the tibia can be seen to articulate with the talus without any intervening fluid (Fig. 53-US13). When an effusion is present, it is seen as a triangular, dark gray to black pocket between the tibia and talus (Fig. 53-US14). Ultrasound can also be used to identify the location of the dorsalis pedis artery before aspiration.

Elbow

The elbow is easily evaluated from the posterior approach. With this approach the transducer is placed over the olecranon fossa with the elbow flexed and the lower part of the arm supported (Fig. 53-US15). In a normal elbow the olecranon fossa can be seen as a slight “divot” between the olecranon of the ulna and the humerus (Fig. 53-US16). When an effusion is present, dark gray or black fluid can be seen to distend this space (Fig. 53-US17).

Hip

The hip joint is unique in that physical examination may suggest the presence of an effusion, but direct confirmation is difficult with traditional examination techniques. Ultrasound will easily confirm the presence of an effusion. To evaluate the hip joint, a low-frequency transducer (3 to 5 mHz) should initially be selected because of the depth of the joint. In very thin patients, the distance from the skin to the joint may be small enough to allow the use of a high-frequency transducer. The transducer should be aligned in a slightly oblique axis (mimicking the orientation of the femoral neck) along the inguinal area. It may be helpful to aim toward the umbilicus. The femoral neck and head should be sought. They will appear as brightly echogenic outlines in the expected shape of the bones (Fig. 53-US18). Once the femur has been identified, the joint capsule should be sought. It will appear as an arcing, hyperechoic line superficial to the bones (Fig. 53-US19). In a normal hip there will be very little tissue between these two structures. There may be a small amount of anechoic or hypoechoic fluid present in this space in a normal hip, and correlation with the unaffected side will aid in evaluation. In the presence of a significant effusion, a large anechoic or hypoechoic fluid collection will be seen between the femoral neck and the capsule (Fig. 53-US20).

Aspiration

Once the joint in question has been evaluated, the optimal site for aspiration can be planned. In contrast to the traditional, blind aspiration technique, the use of ultrasound may suggest an alternative approach. Ultrasound will enable the clinician to map the best approach to the effusion. Once this approach has been clarified, one of two techniques can be applied. The site can be marked and the aspiration can then proceed blindly under sterile conditions. In other cases it may be preferable to perform the tap under direct ultrasound guidance. In these circumstances the needle is inserted either from the transverse or from the long-axis approach and guided directly into the joint space.

General Arthrocentesis Technique

Joint fluid may be obtained even when there is little clinical evidence of an effusion. Although one may aspirate successfully at the point where the joint bulges maximally, certain landmarks are important. The most crucial part of arthrocentesis is defining the joint anatomy by palpating the bony landmarks as a guide. A puncture site and an approach to the joint should be selected; tendons, major vessels, and major nerve branches should be avoided. In most instances the approach is via the extensor surfaces of joints because most major vessels and nerves are found beneath the flexor surfaces. Also, the synovial pouch is usually more superficial on the extensor side of a joint. Ultrasound may be particularly helpful in locating small effusions.

Aseptic technique, including the use of sterile gloves and instruments, is essential to avoid infection. Arthrocentesis should not be attempted if there is a definite or suspected infection overlying the joint. Antiseptic preparation solution should be allowed to dry for several minutes because the bactericidal effects of iodine are both concentration and time dependent. Iodine solution is then removed with an alcohol sponge to prevent transference of iodine into the joint space, which can lead to an inflammatory process. Although the utility of draping is unproved and it may obscure the site, a sterile perforated drape may be placed over the joint.

With appropriate local anesthesia, arthrocentesis should be a relatively painless procedure; without anesthesia, it may be quite painful and distressing to the patient. The synovial membrane itself has pain fibers associated with blood vessels, and the articular capsule and periosteum are richly supplied with nerve fibers and are very sensitive. The articular cartilage has no intrinsic pain fibers. It is important to have the patient relax during the procedure. Tense muscles narrow the joint space and make the procedure more difficult, often necessitating repeated attempts or resulting in inadequate drainage. Distraction of the joint may enhance the target area, especially in areas such as the wrist and finger joints. Traction not only increases the chance of entering the joint but also lessens the chance of scoring the articular cartilage with the needle.

Anesthesia is best accomplished by infiltrating the skin down to the area of the joint capsule along the entire route of needle penetration with a local anesthetic agent such as 1% or 2% lidocaine (Xylocaine) via a 25- to 27-gauge needle. For extremely painful joints, a regional nerve block is appropriate.

The landmarks described in “Specific Arthrocentesis Techniques” later in this chapter should be used and care taken to not bounce the needle off bony structures as a means of finding the joint space because this may cause unnecessary pain. However, in contrast to earlier beliefs, striking bone with the arthrocentesis needle is unlikely to damage articular cartilage.2 An 18- to 22-gauge needle or intravenous catheter and needle set of suitable length attached to an appropriately sized syringe is inserted at the desired anatomic point through the skin and subcutaneous tissue into the joint space. The largest needle that is practical is used to avoid obstructing the lumen with debris or clot. In large joints such as the knee, which can accommodate large effusions, it is suggested that one use a 30- to 60-mL syringe because it may be difficult to change a syringe when the needle is within the joint cavity (Fig. 53-5). A three-way stopcock placed between the needle and the syringe is an option for draining large effusions (Fig. 53-6). If the syringe must be changed during the procedure, the hub of the needle should be grasped with a hemostat and held tightly while the syringe is removed. The authors prefer to use only a rigid needle and not a flexible catheter to perform arthrocentesis; however, a sturdy catheter is used by some clinicians. If an intravenous catheter and needle set is used, the needle is removed while leaving the outer atraumatic plastic catheter in the joint space. The syringe is then attached to the catheter for aspiration. Manipulation of the joint or catheter can now occur with little threat of tissue injury.

Aspiration of synovial fluid and easy injection and return of fluid indicate intraarticular placement of the needle tip. As a general rule, one should try to remove as much fluid or blood as possible. If the fluid stops flowing, it indicates that the joint has been drained completely, the tip of the needle has become dislodged, or debris or clot is obstructing the needle. One should slightly advance or retract the tip of the needle, rotate the bevel, or lessen the force of aspiration. One should never reintroduce a needle through a plastic catheter that has been left in the joint. Occasionally, reinjecting a small amount of fluid into the joint space confirms placement of the needle and may clear the needle. If fluid flows freely back into the joint and is easily reaspirated, one has probably removed all the fluid. If resistance is met, the needle has probably been jarred from the joint space and is lodged in soft tissue. In some instances, minor changes in position produced by flexion or extension of the joint may allow the fluid to flow more freely. Scraping or shearing the articular cartilage with the needle should be avoided. One should enter the joint in a straight line and avoid unnecessary side-to-side motion of the needle.

Synovial fluid should be sent for studies as indicated by the clinical situation. Studies usually obtained include cell count with differential, crystal analysis, Gram staining, and bacterial culture and sensitivity analysis. Synovial protein, glucose, and lactate dehydrogenase determinations have been shown to be unreliable in distinguishing noninflammatory from inflammatory and infectious causes and are no longer recommended.2,2933 Less frequently obtained studies include rheumatoid factor analysis, lupus erythematosus cell preparation, viscosity analysis, mucin clot, fibrin clot, fungal and acid-fast stains, Lyme titer, fungal and tuberculous culture, and synovial fluid complement analysis. If the arthrocentesis is performed for the relief of hemarthrosis, the fluid need not be sent for analysis. One should be selective in ordering tests. There is no need to order a large battery of tests routinely on all fluids. If the volume of fluid collected is low, Gram stain, culture, and examination of the “wet preparation” under regular and polarizing microscopy have the highest priority. Prompt examination of specimens should be performed to avoid misdiagnosing borderline inflammatory fluids, missing crystals that dissolve with time, or overinterpreting the findings because of new artifactual crystals that appear over a prolonged time.34

Complications

Significant complications are rare with arthrocentesis but include the following:

1. Infection. Skin bacteria may be introduced into the joint space during needle puncture. Nevertheless, infection occurs rarely because the bacteria are either quickly cleared or not viable.2 One can further limit this complication by maintaining rigorous sterile technique and avoiding insertion of the needle through obviously (or possibly) infected skin or subcutaneous tissue. Various studies report the incidence of infection after routine arthrocentesis to be in the range of 1 in 10,000.34 However, in immunocompromised patients, particularly those with rheumatoid arthritis, the incidence is higher (1 in 2000 to 10,000 aspirations).35,36 Joint aspiration in the presence of bacteremia was discussed previously. Acute pain, redness, and swelling 12 to 24 hours after steroid injection can mimic infection but is most likely an inflammatory reaction (steroid flare) to the steroid preparation (often methylprednisolone).

2. Bleeding. Bleeding with subsequent hemarthrosis is rarely a complication, except in patients with a bleeding diathesis. In those with a bleeding diathesis such as hemophilia, arthrocentesis should be delayed until clotting competence has been enhanced by infusing specific clotting factors. In general, spontaneous bleeding into a hemophiliac’s joint is an indication for replacement of clotting factors. Occasionally, a small quantity of blood may be aspirated along with synovial fluid. This happens most often when the joint is nearly emptied. A small amount of blood-tinged fluid is generally the result of nicking a small synovial blood vessel; it is usually inconsequential.

    Arthrocentesis and joint injections in patients receiving chronic warfarin therapy, with a therapeutic INR, were shown to be safe by Ahmed and Gertner,37 without an increased risk of bleeding complications. In this study of 456 procedures in patients on chronic warfarin therapy, there was no statistically significant difference in the overall complication rate between patients with an international normalized ratio 2.0 or greater and patients with an international normalized ratio less than 2.0. Of note, 103 of 456 procedures (22.5%) were safely performed in patients with an international normalized ratio greater than 3, with the highest international normalized ratio being 7.8.

3. Allergic reaction. Hypersensitivity to the local anesthetic can usually be prevented by thorough history taking. Facial and torso flushing associated with corticosteroid injection may represent an idiosyncratic reaction to preservatives in the steroid preparation.2 Fainting during the procedure is not uncommon and most often the result of vasovagal influences.

4. Corticosteroid-induced complications. See “Intraarticular Corticosteroid Injections,” earlier.

Specific Arthrocentesis Techniques

Arthrocentesis of the hip is generally performed by an orthopedic surgeon under fluoroscopic, ultrasound, magnetic resonance imaging, or computed tomography guidance and is not discussed here. If available, fluoroscopy or ultrasound can also be used to guide aspiration of other joints, but these imaging adjuncts are not generally required. For small joints, application of traction is often very helpful in obtaining fluid. While applying continuous suction to the aspirating syringe, walk the needle over palpated bone until the joint is entered or fluid is obtained. However, it may be quite difficult to obtain fluid from small joints in the hand and foot, and the clinician must often treat empirically. If only one drop of fluid is obtained from small joints, it is best to send it for culture.

First Carpometacarpal Joint (Fig. 53-7)

Interphalangeal and Metacarpophalangeal Joints (Fig. 53-8)

Radiocarpal Joint (Wrist) (Fig. 53-9)

Radiohumeral Joint (Elbow) (Fig. 53-10)

Landmarks.: The lateral epicondyle of the humerus and the head of the radius are the arthrocentesis landmarks for the radiohumeral joint. With the elbow extended, palpate the depression between the radial head and the lateral epicondyle of the humerus.

Comments.: Elevation of the anterior fat pad or the presence of a posterior fat pad on a lateral soft tissue elbow radiograph signifies blood, pus, or fluid in the elbow joint (see Fig. 53-10B). Effusions in the elbow joint may bulge and be readily palpated (see Fig. 53-10C). Frequently, the effusion appears inferior to the lateral epicondyle. The bulge can then be aspirated from a posterior approach on the lateral side (see Fig. 53-10D). A medial approach is not recommended because the ulnar nerve and superior ulnar collateral artery may be damaged. Gout and septic arthritis commonly affect this joint. The most common cause of elbow hemarthrosis after trauma with no obvious fracture is a nondisplaced radial head fracture. A small hemarthrosis need not be aspirated, but removal of blood from a tense elbow joint will significantly hasten recovery and facilitate range of motion in patients with a radial head fracture.

Knee Joint, Anteromedial Approach (Fig. 53-12)

Comments.: If the patient is tense, contraction of the quadriceps will greatly hinder entering the joint. However, the knee is probably the easiest joint to enter, and removal of a tense hemarthrosis will relieve pain and facilitate examination for ligamentous injury. If fluid stops flowing, the operator or assistant should squeeze the soft tissue area of the suprapatellar region to “milk” the suprapatellar pouch of fluid. Alternatively, wrap the patient’s thigh with a 6-inch elastic bandage from the groin to the suprapatellar area before beginning the procedure. The knee can easily accommodate 50 to 70 mL of fluid, and the clinician should therefore use a large syringe. Holding or securing the hub of the needle with a hemostat allows the clinician to remove the syringe without changing the position of the intraarticular needle. Alternatively, a stopcock on the needle will allow complete removal of fluid without changing the position of the needle (see Fig. 53-12B). The knee is a common site for septic arthritis (especially gonococcal) and various inflammatory or degenerative diseases. An anterolateral approach can be accomplished in a similar manner.

Tibiotalar Joint (Ankle) (Fig. 53-13)

Metatarsophalangeal and Interphalangeal Joints (Fig. 53-14)

Landmarks.: For the first digit, landmarks are the distal metatarsal head and the proximal base of the first phalanx. For the other toes, the landmarks are the prominences at the proximal interphalangeal and distal interphalangeal joints. The extensor tendon of the great toe can be located by active extension of the toe.

Synovial Fluid Interpretation

Synovial fluid examination is essential for the diagnosis of septic arthritis, gout, and pseudogout.3840 Inflammatory joint disease of previously unknown etiology can often be diagnosed precisely by synovial fluid analysis. Joint fluid is a dialysate of plasma that contains protein and hyaluronic acid. Normal fluid is clear enough to read newsprint through and will not clot. It is straw colored, flows freely, and has the consistency of machine oil. Normal fluid produces a good mucin clot and yields a positive “string sign” (see the next section). The uric acid level of joint fluid approaches that of serum, and the glucose concentration is normally at least 80% of that in serum. Clarity of fluid reflects the leukocyte count. High leukocyte counts result in opacity, the degree of which generally correlates with the degree of elevated synovial fluid leukocytes. However, the degree of opacity cannot be used to reliably determine the synovial fluid leukocyte count and should not be used as a surrogate for laboratory cell count measurements.

String Sign

Viscosity correlates with the concentration of hyaluronate in synovial fluid. Any inflammation degrades hyaluronate, which characteristically results in low-viscosity synovial fluid. The string sign is a simple test for assessing viscosity. The practitioner measures the length of the “string” formed by a falling drop of synovial fluid extruded from a syringe or stretched between the thumb and the index finger of a gloved hand. Normal joint fluid produces a string 5 to 10 cm long (Fig. 53-15). If viscosity is reduced, as with inflammatory conditions, synovial fluid forms a shorter string or falls in drops.

Cell Count

A leukocytosis consisting predominantly of neutrophils is usually seen with inflammatory arthritides; a WBC count greater than 50,000/mm3 (i.e., >50,000/µL) is highly suggestive of a septic joint. Shmerling and colleagues38 found a WBC count of greater than 2000/mm3 to be 84% sensitive and 84% specific for all inflammatory arthritides. Of their septic arthritis patients, 37% had a synovial WBC count lower than 50,000/mm3. However, 89% of their patients with a synovial WBC count greater than 50,000/mm3 had a septic joint.38

Glucose and Protein

Current literature suggests that synovial protein and glucose are highly inaccurate markers of inflammation.2,28 In one study of 100 consecutive patients undergoing diagnostic arthrocentesis, the sensitivity of synovial protein and glucose was found to be 0.52 and 0.20, respectively.36 The authors of this study recommended that ordering chemistry studies on synovial fluid should be discouraged because such studies are likely to provide misleading or redundant information.

Serology

Though available, most serologic tests are not likely to be useful in the emergency setting. Polymerase chain reaction (PCR) is an effective means of identifying septic arthritis, even in the setting of a negative fluid culture or when antibiotics have been administered previously. PCR can also help isolate slowly growing microorganisms. Gas-liquid chromatography, a rapid and sensitive method for detection of short-chain fatty acids, may complement the currently available methods used to diagnose septic arthritis.39

Counterimmunoelectrophoresis and latex agglutination are also useful and available in some centers on an emergency basis. Other immunologic markers such as complement, rheumatoid factor, and antinuclear antibodies have little diagnostic value in the acute setting but may be useful to the clinician providing follow-up care when compared with serum levels.

Polarizing Microscope

No synovial fluid analysis is complete until the fluid has been examined under a polarizing light microscope for crystals. The polarizing microscope used for crystal identification differs from the ordinary light microscope in that it contains two identical polarizing prisms or filters. One filter, called the polarizer, is positioned below the condenser. The other filter is called the analyzer and is inserted at some point above the objective. Examination for crystals is performed by most hospital laboratories.

Microscopic Analysis

When examining crystals under polarized microscopy, the technician orients crystals on a stage according to two axes, referred to as x and z. If the long axis of the crystals is blue when parallel to the z-axis and yellow when perpendicular to it, it is calcium pyrophosphate and termed positively birefringent. If the long axis of the crystal is yellow when parallel to the z-axis and blue when perpendicular to it, it is monosodium urate and termed negatively birefringent. Urate crystals are 2 to 10 µm and usually needle shaped (Fig. 53-16). Calcium pyrophosphate crystals range from 10 µm down to tiny crystals that have to be examined with the oil objective; they appear as rods, rhomboids, plates, or needle-like forms and are weakly birefringent (Fig. 53-17). Cholesterol crystals are sometimes seen and are large, very bright square or rectangular plates with broken corners.40,41

Items found in synovial fluid that can be confused with sodium urate or calcium pyrophosphate crystals include collagen fibrils, cartilage fragments, cholesterol crystals, metallic fragments from prosthetic arthroplasty, and corticosteroid esters.40 One may also identify fat globules (Fig. 53-18). Note that rare cases of uric acid spherulites in gouty synovia have been reported.40 The spherulites are birefringent and do not take up fat stains.

Table 53-2 summarizes synovial fluid features for the joint diseases commonly encountered and studies commonly performed in the ED.

TABLE 53-2

Characteristics of Synovial Fluid

image

PMN, polymorphic nuclear cell; PVNS, pigmented villonodular synovitis.

Adapted from Harris ED, Budd RC, Genovese MC, et al, eds. Kelley’s Textbook of Rheumatology. 7th ed. Philadelphia: Elsevier; 2005.

Joint Arthrography

Indications and Contraindications

SA should be performed in patients with penetrating injuries near a joint in which violation of the joint itself is unclear (Fig. 53-19). Smaller joints such as those in the hand are inspected visually. However, for larger joints, SA is the preferred test.

Contraindications to performing SA are essentially the same as for performing arthrocentesis. When indicated, underlying fracture of the joint should first be ruled out. An obvious open fracture would preclude the need to perform SA.

Equipment and Procedure

Aseptic technique is essential, but the equipment and procedure are essentially the same as for performing arthrocentesis, with minor differences. First, a source of sterile saline is required (e.g., a small bag of intravenous fluid). Second, larger joints require more saline infusion, and this is most easily performed if a stopcock is used to allow refill of the syringe (Fig. 53-20).

Because saline is not as viscous as joint fluid, a 20-gauge needle is sufficient. Once the joint space has been reliably entered, a variable amount of saline to “load” that joint is slowly injected. The amount of saline injected varies with the size of the joint. In general, a sufficient volume should be injected to visibly distend the joint or create resistance to injection and cause the patient discomfort. The sensitivity of the test in detecting small traumatic joint injuries is proportional to the volume injected. Specifically, for knee injuries, injecting 50 mL of saline was 46% sensitive and injecting 100 mL, 75% sensitive; to achieve 95% sensitivity required an average of 194 mL of saline.43 The following is the recommended volume of injection per joint:

Once the injection is complete, do not remove the needle but close the stopcock to avoid backflow. Examine the joint for evidence of leakage of fluid from the wound. This is performed in a static position but, if negative, also with some gentle passive movement of the joint. Visible leakage of fluid into the laceration confirms the diagnosis of joint space violation. A negative test is defined as absence of evidence of leakage after an appropriate amount of saline has been injected. A slow loss of fluid may indicate a small insult to the joint, and saline can be left in the joint for a few minutes to observe for this. After completion, the fluid should be evacuated for patient comfort. This is generally performed by leaving the original needle in place with a closed stopcock attached, which is then used to aspirate the saline in the joint.

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