Urinary tract infections

Published on 02/03/2015 by admin

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Last modified 02/03/2015

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36 Urinary tract infections

Key points

The term urinary tract infection (UTI) usually refers to the presence of organisms in the urinary tract together with symptoms, and sometimes signs, of inflammation. However, it is more precise to use one of the following terms.

Epidemiology

UTIs are among the most common infectious diseases occurring in either the community or health care setting. Uncomplicated UTIs typically occur in healthy adult non-pregnant women, whereas complicated UTIs are found in either sex and at any age, frequently associated with structural or functional urinary tract abnormalities.

Pathogenesis

There are three possible routes by which organisms might reach the urinary tract: the ascending, blood-borne and lymphatic routes. There is little evidence for the last route in humans. Blood-borne spread to the kidney can occur in bacteraemic illnesses, most notably Staphylococcus aureus septicaemia, but by far the most frequent route is the ascending route.

In women, UTI is preceded by colonisation of the vagina, perineum and periurethral area by the pathogen, which then ascends into the bladder via the urethra. Uropathogens colonise the urethral opening of men and women. That the urethra in women is shorter than in men and the urethral meatus is closer to the anus are probably important factors in explaining the preponderance of UTI in females. Further, sexual intercourse appears to be important in forcing bacteria into the female bladder, and this risk is increased by the use of diaphragms and spermicides, which have both been shown to increase E. coli growth in the vagina. Whether circumcision reduces the risk of infection in adult men is not known, but it markedly reduces the risk of UTI in male infants.

Clinical manifestations

Most UTIs are asymptomatic. Symptoms, when they do occur, are principally the result of irritation of the bladder and urethral mucosa. However, the clinical features of UTI are extremely variable and to some extent depend on the age of the patient.

Investigations

The key to successful laboratory diagnosis of UTI lies in obtaining an uncontaminated urine sample for microscopy and culture. Contaminating bacteria can arise from skin, vaginal flora in women and penile flora in men. Patients therefore need to be instructed in how to produce a midstream urine sample (MSU). For women, this requires careful cleansing of the perineum and external genitalia with soap and water. Uncircumcised men should retract the foreskin. This is followed by a controlled micturition in which about 20 mL of urine from only the middle portion of the stream is collected, the initial and final components being voided into the toilet or bedpan. Understandably, this is not always possible and many so-called MSUs are in fact clean-catch specimens in which the whole urine volume is collected into a sterile receptacle and an aliquot transferred into a specimen pot for submission to the laboratory. These are more likely to contain urethral contaminants. In very young children, special collection pads for use inside nappies or stick-on bags are useful ways of obtaining a urine sample. Occasionally, in-and-out diagnostic catheterisation or even suprapubic aspiration directly from the bladder is necessary.

For primary care doctors located some distance from a laboratory, transport of specimens is a problem. Specimens must reach the laboratory within 1–2 h or should be refrigerated; otherwise, any bacteria in the specimen will multiply and might give rise to a false-positive result. Methods of overcoming bacterial multiplication in urine include the addition of boric acid to the container and the use of dip-slides, in which an agar-coated paddle is dipped into the urine and submitted directly to the laboratory for incubation. Both of these alternatives have difficulties. For the boric acid technique, it is important that the correct amount of urine is added to the container to achieve the appropriate concentration of boric acid (1.8%, w/v), as the chemical has significant antibacterial activity when more concentrated. When the dip-slide is used, no specimen is available on which to do cell counts.

Concerns about the relative expense and slow turn-around time of urine microscopy and culture have stimulated interest in alternative diagnostic strategies. Some advocate a policy of empirical antimicrobial treatment in the first instance, and reserve investigation only for those cases that do not respond. Others are in favour of using cheaper, more convenient screening tests, for example, urine dipsticks. It is important to be aware that there is no rapid screening test that will reliably detect all UTIs. Urine microscopy and culture remain the standard by which other investigations are measured.

Dipsticks

Dipsticks for rapid near-patient testing for urinary blood, protein, nitrites and leucocyte esterase are usually used, although there are concerns that these are reliable only when applied to fresh urine samples tested at the point of care. Assessment of colour changes on dipsticks can be subjective and automated reading systems have been developed to assist interpretation. Generally, the negative predictive value is better than the positive predictive value, so their preferred use is as screening tests to identify those specimens which are least likely to be infected and which therefore do not require culture. A perfectly valid alternative is just to hold the specimen up to the light: specimens that are visibly clear are very likely to be sterile.

The leucocyte esterase test detects enzyme released from leucocytes in urine and is ∼︀90% sensitive at detecting white blood cell counts of >10 mm−3. It will be positive even if the cells have been destroyed due to delays in transport to a laboratory. However, vitamin C and antibiotics in the urine such as cephalosporins, gentamicin and nitrofurantoin may interfere with the reaction. Although the presence of leucocytes is common in UTIs, it may also occur in other conditions. Particularly in children, white blood cells can be present for many reasons, including fever alone.

The nitrite test (also called the Griess test) detects urinary nitrite made by bacteria that can convert excreted dietary nitrate used as a food preservative to nitrite. Although the coliform bacteria that commonly cause UTI can be detected in this way, some organisms cannot, for example, enterococci, group B streptococci, Pseudomonas