CHAPTER 94 PARASITIC AND FUNGAL INFECTIONS
PARASITIC INFECTIONS OF THE CENTRAL NERVOUS SYSTEM
In 1909 John D. Rockefeller declared, “Deprivation in the agriculturally rich Southern States is not due to stupidity or laziness, but to parasite infestation.”1 The human immunodeficiency virus pandemic and global warming have resurrected the study of parasitology. In general, parasitic diseases are treatable and should be considered when geography, patient susceptibility, and exposure make infection possible.
More than 2 million people die each year of falciparum malaria; 200 million are infected with schistosomiasis. Toxoplasma species flourish in patients with acquired immunodeficiency syndrome (AIDS). Epilepsy from neurocysticercosis affects more than 1% of the population in some regions. Most cases of neurocysticercosis in Mexico remain undiagnosed despite the people having cognitive and psychiatric problems throughout life. Sleeping sickness affects 300,000 Africans and threatens many more. Intestinal helminths dull children’s minds.2
This chapter addresses the major neuroparasites, as is customary in the neurosciences, by location and clinical presentation. The aim is to present the clinician with a catalog of possibilities so that a treatable disease is not overlooked (Table 94-1).
CSF, cerebrospinal fluid; MRI, magnetic resonance imaging.
Amebic Encephalitis
Various features of primary amebic meningoencephalitis (a fulminant encephalitis) and granulomatous amebic encephalitis (a subacute granulomatous disease) are summarized in Table 94-2.
Feature | PAM | GAE |
---|---|---|
Risk factors | Swimming | Diabetes, pregnancy, alcohol/cirrhosis, corticosteroids, AIDS, chemotherapy/radiotherapy |
Organism | Naegleria | Acanthamoeba27,28 |
Balamuthia | ||
Route to CNS | Olfactory epithelium/nerves | Intranasal/intracranial vasculitis leading to thrombosis and infarction |
CNS disease | Culture-negative, fulminant, purulent meningitis; mostly PMN, unlike TB or viral | Focal or diffuse encephalitis with meningism, giant cell reaction29 |
Organism can harbor | Yes30 | Yes |
Legionella, Vibrio cholerae | ||
CSF laboratory findings | Glucose variable; protein >1 g/L | Glucose variable |
CSF microscopic findings | Motile Naegleria move 1-3 body lengths/min11 | Lymphocytic pleocytosis; trophozoite seldom found in CSF31,32 |
AIDS, acquired immunodeficiency syndrome; CNS, central nervous system; CSF, cerebrospinal fluid; GAE, granulomatous amebic encephalitis; PAM, primary amebic meningoencephalitis; PMN, polymorphonuclear neutrophils; TB, tuberculosis.
Entamoeba histolytica
Diagnosis
Diagnosis is by use of microscopy after staining tissue with Gomori trichrome or the iron hematoxylin method. The sensitivity of microscopy is low. The organism may not be found in stool. Enzyme-linked immunosorbent assay (sensitivity, 87%) and polymerase chain reaction (sensitivity, 85%) are used on stool or serum.34–36
African Trypanosomiasis
Epidemiology
David Livingstone first attributed nagana to the tsetse fly (Glossina) in 1857. The disease is transmitted by male and female tsetse flies through a bite that is painful and does not go unnoticed. Trypanosoma brucei gambiense is found in West Africa; the infection takes months or years to affect the central nervous system (CNS). Trypanosoma brucei rhodesiense causes an acute illness in East Africa, affecting the brain within a few months of the original bite. Transplacental transmission also occurs.
Diagnosis
T. b. rhodesiense, however, can be found in blood. Concentration techniques are used for blood and cerebrospinal fluid (CSF) samples. Inoculation of rodents, which are killed after 21 days, is useful for identification of T. b. rhodesiense, but serological results are positive only after the onset of clinical symptoms, limiting its use in the asymptomatic traveler who is bitten in an endemic area where cattle are dying.37
Treatment
If CSF is positive for leukocytes and IgM concentrations are high, melarsoprol (a trivalent arsenic compound) is the drug of choice (Table 94-3). Relapse rates are high, and up to 20% of patients infected with T. b. gambiense do not respond to melarsoprol. Eflornithine is an alternative therapy for infections from T. b. gambiense but not from T. b. rhodesiense. Melarsoprol may cause post-treatment reactive encephalopathy in up to 10% of cases, with a mortality rate of 50%. Thus, the overall mortality from melarsoprol is 5% from arsenic encephalopathy.
TABLE 94-3 Treatment of Trypanosoma brucei gambiense and Trypanosoma brucei rhodesiense Sleeping Sickness*
Stage | West African (T. b. gambiense) | East African (T. b. rhodesiense) |
---|---|---|
Early stage | ||
Endemic countries | According to national legislation or guidelines | According to national legislation or guidelines |
Other countries | Pentamidine isethionate, 4 mg/kg body weight daily or on alternate days for 7 to 10 days intravenously† or intramuscularly | Suramin, test dose of 4-5 mg/kg body weight at day 1, followed by 5 injections of 20 mg/kg body weight every 7 days (e.g., days 3, 10, 17, 24, 31); the maximum dose per injection is 1 g |
Late stage | ||
Endemic countries | According to national legislation or guidelines | According to national legislation or guidelines |
Other countries | If available: eflornithine, 100 mg/kg body weight at 6-hour intervals for 14 days (150 mg/kg body weight in children) by short infusions over a period of at least 30 minutes | Eflornithine not recommended (low efficacy38) |
Alternative: melarsoprol,‡ 3 series of 3.6 mg/kg body weight at 24-hour intervals for 3 days; the series are spaced by intervals of 7 days | Melarsoprol,‡§ 1 series of 1.8, 2.16, 2.52 mg/kg body weight at 24-hour intervals; 1 series of 2.52, 2.88, 3.25 mg/kg body weight at 24-hour intervals; and 1 series of 3.6, 3.6, 3.6 mg/kg body weight at 24-hour intervals; the series are spaced by intervals of 7 days (the maximum dose is 5 mL) |
* No firm recommendations exist for the use of the trypanocidal drugs; the schedules indicated are those most commonly used. A concise treatment schedule for treatment of T. b. gambiense sleeping sickness consisting of 10 days of melarsoprol, 2.2 mg/kg body weight daily, is under final evaluation.39,40 Note: This 10-day schedule must not be used for treatment of T. b. rhodesiense sleeping sickness, because there are no data available.
† Very slow intravenous injection or short infusion, only under well-controlled circumstances.
‡ The concomitant application of 1 mg/kg body weight prednisolone has been shown to reduce the incidence of encephalopathic syndromes in one large-scale clinical trial.41
§ A single dose of Suramin is often applied before the stage determining lumbar puncture.
From Burri C, Brun R: Human African trypanosomiasis. In Cook GC, Zumla AI, eds: Manson’s Tropical Diseases, 21st ed. Edinburgh: Saunders, 2003, pp 1303-1323. Used with permission.
A lumbar puncture should be performed 1 day after a course of therapy for late-stage disease. All patients should be monitored for 2 years with lumbar punctures every 6 months. A relapse is suspected if the CSF cell count is more than 20 cells/μL. Reinfection is likely when CSF has more than 50 cells/μL, when the count has doubled since the previous count, or when there are 20 to 49 cells/μL in a symptomatic patient.25,39
Angiostrongylus cantonensis
Epidemiology
Angiostrongylus cantonensis is found mostly in southeast Asia and the Pacific Basin, but its distribution is spreading to Africa and the Caribbean. The infection is mainly acquired by eating raw or undercooked snails or slugs; it may also be acquired from eating crabs and freshwater shrimp.
Cysticercus of Taenia solium
Epidemiology
Humans are an accidental end-stage host of cysticerci, which die and degenerate in the brain, eye, and spinal cord, causing an inflammatory reaction. Cysticercosis is the most common cause of epilepsy in developing countries. T. solium intestinal carriers, releasing up to 200,000 eggs per day into their environment, are infective sources of cysticercosis, endangering all who come in contact with them. In 1993, the World Health Organization declared cysticercosis a potentially eradicable disease by tracing human carriers of T. solium. In the early 19th century, cysticercosis was diagnosed in 2% of autopsies in Berlin, roughly the same rate as found today in Mexico.11 The Commission on Tropical Diseases of the International League Against Epilepsy states that the prevalence of active epilepsy in tropical countries ranges from 1% to 1.5%, almost twice the level in Western countries. Regions and peoples with a religious aversion to pork are spared.24,43,44
Clinical Presentation
With parenchymal disease, most children (80%) present with seizures. Episodic headaches and vomiting are featured in one third. Ventricular cysts may wander in the isodense CSF and cause hydrocephalus through an intermittent ball-valve effect. Subarachnoid cysts can result in meningeal signs, usually without fever. Rarely, cysticerci erode into large vessels. Arachnoiditis is another cause of hydrocephalus, as well as focal vasculitis, which may be responsible for lacunar strokes. Small space-occupying lesions are responsible for focal weakness. Cognitive and psychiatric impairments are common. In the eye, a scotoma may develop or vision may be lost.45
Diagnosis
Blood and CSF serological analyses have improved. Many clinicians use the enzyme-linked immunoelectrotransfer blot (EITB) assay. It derives from coproantigens of adult T. solium and ignores nonspecific bands of antigen. EITB results may revert to negative after the cysticercus dies. The assay is not always readily available. In the blood, sensitivity is 92.5% and specificity is 100%. However, EITB sensitivity has been reported to be as low as 28% in subjects with a single parenchymal lesion, as is commonly seen in India.46 EITB sensitivity may be less in CSF.47
The diagnosis requires neuroimaging, with the goal of identifying the scolex (2 to 3 mm). Only since the frequent use of computed tomography in the 1980s has cysticercosis emerged as the most common cause of epilepsy in many areas. (Cerebral malaria is likely the most common cause of febrile seizures.) Magnetic resonance imaging may show a diagnostic invaginated scolex but not show the calcific stage, for which computed tomography is best. Magnetic resonance imaging can show ventricular cysts. In brain parenchyma, the cysts mature in 3 months to 10mm in size (occasionally up to 20mm); in the ventricles, they can exceed 5 cm. Therefore, serum EITB, CSF ELISA, and neuroimaging have become useful diagnostic tools48,49 (Figs. 94-1 through 94-7).
Treatment





Figure 94-8 Antiepileptic treatment for patients with first seizure due to neurocysticercosis.52 Asterisk indicates that “degenerative” includes a single enhancing lesion on computed tomography (computed tomography) after differential diagnosis has been established. AEM, antiepileptic medication; MRI, magnetic resonance imaging.
(From Carpio A: Neurocysticercosis: an update. Lancet Infect Dis 2002; 2:751-762. Used with permission.)
Falciparum Malaria
Pathophysiology


Clinical Presentation
Severe falciparum malaria is clearly defined and should be treated parenterally initially (Table 94-5). Patients with cerebral malaria present with coma, convulsions, dysconjugate gaze, pouting, and bruxism. Retinal hemorrhages are seen. CNS findings are symmetrical. The plantar reflexes are upgoing.68
Cerebral malaria, defined as unrousable coma (lasting >1 hour after a seizure) not attributable to any other cause in a patient with falciparum malaria
|
Modified from the World Health Organization: Management of Severe Malaria: A Practical Handbook, 2nd ed. Geneva: World Health Organization, 2000, p 5. Used with permission.
In children with febrile convulsions followed by coma, 30 to 60 minutes may have to pass before malaria is diagnosed, but treatment should not be delayed. Patients with malarial convulsions may present with only nystagmus, salivation, and twitching of the mouth or a digit. One third of seizures in children manifest only as eye deviation or salivation, or both. One in 10 children with severe malaria has sequelae: ataxia, hemiparesis (hemiconvulsion-hemiparesis syndrome), speech disorder, blindness, behavioral problems, cognitive difficulties (executive functions), and spasticity. Prolonged seizures, coma, hypoglycemia, and absence of hyperpyrexia are associated with neurological sequelae.69
Treatment
Common errors in treating malaria are the following:
Treatment guidelines with quinine and quinidine for severe malaria are as follows:





The artemisinins clear parasitemia faster than quinine:


Corticosteroids are associated with an increased risk of adverse side effects and do not decrease mortality. The diagnosis and management of severe malaria70 and therapeutic options are discussed in other publications.25,70–76
Gnathostoma spinigerum
Epidemiology
Gnathostoma spinigerum, a nematode larva, is acquired in Southeast Asia, Japan, and Latin America from eating undercooked fish, frogs, snakes, ducks, and chicken. Ceviche and sushi are known sources of infection.
Clinical Presentation
One to 2 days after the larva is ingested, it causes epigastric pain as it penetrates the gastric mucosa. A retrospective study of 946 cases of gnathostomiasis in Mexico City identified nodular migratory panniculitis over the trunk as the most common skin sign.77 Migrating immature worms move through spinal foramina at all levels, causing sudden, severe radicular pain and nerve palsy. This resolves in a few days as the worm races to the brain, causing symptoms for as long as 15 years. Focal symptoms are present as well as eosinophilic meningitis with myeloencephalitis, and 25% of the CNS cases are fatal.
Diagnosis
Eosinophilia is prominent and serum levels of IgE are elevated. Diagnosis is by serology (ELISA when available: sensitivity, 93%; specificity, 96.7%) and magnetic resonance imaging (cervical cord enlargement and hemorrhagic tracts in the brain with scattered deep intracerebral hemorrhages). Microscopic examination is diagnostic.78
Paragonimus
Clinical Presentation
The lung infection can be mistaken for bronchiectasis. It may be misdiagnosed as nocardiosis, tuberculosis, or metastatic lung cancer. Symptoms may be recognized for years before diagnosis.79 Epilepsy occurs in 65% to 95% of CNS cases before diagnosis. Chronic mild insidious eosinophilic meningitis (the majority of cases), or recurrent acute meningitis, as well as chronic decline in mental status persisting for as long as 20 years is described. Homonymous hemianopia is common.80,81 Flukes in the spinal cord cause spastic paraplegia.82
Diagnosis
Chest radiographic abnormalities are seen in 80% of patients. Oh83 reviewed 62 cases of cerebral paragonimiasis and found CSF eosinophilia in 8% and peripheral eosinophilia in 41%. If available, serology by enzyme-linked immunosorbent assay and complement fixation on CSF samples may be used for diagnosis and for monitoring therapy. Microscopic examination is performed on stool, gastric washings, sputum, or tissue.84
In chronic brain syndrome, magnetic resonance imaging shows multiple conglomerated, round, enhancing nodules (“soap bubbles”) with encephalomalacia in the temporal, parietal, and occipital lobes. Computed tomography scans show large calcified nodules or multiple cystic calcifications in the same region in 40% to 70% of cases.20,80,82,83
Schistosoma japonicum
Diagnosis
Stool microscopy is performed. Immunodiagnosis is similar to that of S. mansoni. On magnetic resonance imaging and computed tomography, there may be focal and asymmetrical findings, such as a dilated ventricle from massive egg embolization. CSF opening pressure is increased. The CSF cell count is increased (5 to 55 monocytes per high-power field), with increased protein and normal glucose concentrations. Partial seizures are more common in the Philippines and China, whereas generalized seizures are more common in Japan85 (Fig. 94-9).
Schistosoma mansoni and Schistosoma haematobium
Epidemiology
Schistosomiasis affects 200 million people in the Middle East, Africa, Brazil, Suriname, Venezuela, and the Caribbean, wherever the freshwater snail vector is found. In CNS disease, S. haematobium can be found in the brain, but S. mansoni and S. haematobium are more commonly found in the spinal cord.
Clinical Presentation
S. mansoni is the most common cause of schistosomal myeloradiculopathy. The second most common is S. haematobium. Radicular symptoms may occur at any stage of the infection but are more likely during early stages, or with mild chronic forms, than during later stages with severe forms of the disease, such as the hepatosplenic stage.86 In Brazil, Nascimento-Carvalho and Moreno-Carvalho87 reported that the most common CNS manifestations in pediatric neuroschistosomiasis due to S. mansoni are paraparesis (55%), urinary retention (53%), and paraplegia (20%).
Diagnosis
CSF eosinophilia with increased concentrations of protein is common. Stool and urine microscopy and rectal biopsy are diagnostic (S. haematobium eggs are easily detected in the urine). S. mansoni is sometimes found in the urine. Multiple techniques allow schistosome antigens to be identified in the serum, urine, and CSF, mostly using ELISA and circulating anodic antigen. An ELISA of the CSF is helpful for suspected CNS infection.88 Magnetic resonance imaging shows an enlarged spinal cord.
Toxoplasma gondii
Epidemiology
Toxoplasma gondii is a protozoan found worldwide from Alaska to Australia. One third of the human population has been exposed. In one third of AIDS patients who are Toxoplasma seropositive, cerebral toxoplasmosis reactivates. Toxoplasmosis sickens fetuses and immunosuppressed hosts. Prophylactic medication has impacted the prevalence of postnatal infection. Nonfetal infection is acquired by ingesting tissue cysts in undercooked meat (pork, lamb, and rabbit) or oocysts in cat feces that have contaminated food, water, or hands (pregnant women should wear gloves when gardening). In British Columbia, a water reservoir became infected from use by cougars. Toxoplasmosis may be transmitted by blood transfusion and organ donation. Immunosuppressive therapy prescribed to transplant patients puts them at higher risk.89
Pathophysiology
CD4 and CD8 cells and possibly astrocytes prevent reactivation in immune individuals. CD4 counts less than 100/μL are associated with more severe infections. TH1 helper T cells produce interleukin 2, interferon γ, and tumor necrosis factors α and β. Interferon γ has a preeminent role in the development of toxoplasmosis. Its synthesis results in chronic asymptomatic infection. When the CD4 cell count is less than 100/μL, interferon γ production decreases or stops. The imbalance between TH1 and TH2 responses allows interleukins 4 and 10 to further downregulate the secretion and activity of interferon γ. With progression of disease, multifocal necrotizing encephalitis develops, particularly in the thalamus, basal ganglia, and the intersection between white and gray matter in the frontal and temporal cortex.90
In dormant stages, bradyzoites are within pseudocysts in brain tissue and skeletal muscle. With reactivation, tachyzoites invade virtually any cell. The parasite favors neurons over microglial cells because they do not express major histocompatibility complex class 1 molecules and cannot be eliminated by sensitized CD8+ T cells.65
Clinical Presentation
Acute toxoplasmosis induces a mononucleosis-like illness. Acute infection or reactivation in the immunosuppressed patient may result in encephalitis and devastating disease. Severe bilateral headache has little response to analgesics. Focal neurological signs are common (69% of cases). Seizures are observed in 15% to 30% as a presenting sign. Depression, bipolar disorder, altered mental status, ataxia, and dementia may follow. Blindness, with mental retardation and intracranial calcification (Table 94-6), and hydrocephalus occur in neonates.
TABLE 94-6 Infectious Causes of Central Nervous System Calcifications
FUNGAL INFECTIONS OF THE CENTRAL NERVOUS SYSTEM
Central Nervous System Cryptococcosis
C. neoformans is a round or oval yeast that has a worldwide distribution and is found particularly in bird excreta.91 Infection is acquired through inhalation of the organism. Most pulmonary infections are asymptomatic, but acute symptomatic pneumonia may occur. Infection can disseminate to distant sites either during this primary lung infection or later during reactivation, usually as a result of cell-mediated immune deficiency.92 The most likely site for dissemination is the CNS, but the reason is not entirely clear. Some investigators have hypothesized that there is a receptor in the CNS that binds a ligand on the organism, but this hypothesis has not been verified.
Patients with CNS cryptococcosis usually present with a subacute meningitis, with signs and symptoms such as headache, fever, and focal neurological deficits occurring over several weeks.93,94 Symptoms may be subtle, waxing and waning. Classic meningeal symptoms (e.g., neck stiffness or photophobia) may not always be present. In particular, in human immunodeficiency virus infection, they are present in only one fourth to one third of patients. Brain imaging studies are also nonspecific; computed tomography scans may be normal in one half the cases. Cerebral atrophy and ventricular enlargement are the most common findings in human immunodeficiency virus–infected patients. Cryptococcomas (nodules in the brain parenchyma) can also be present occasionally, especially in patients with infection caused by C. neoformans var. gattii. CSF findings usually include an increased opening pressure, mild mononuclear pleocytosis, and increased protein concentration. India ink examination of the CSF is positive in 50% of non-AIDS patients with cryptococcal meningitis and in more than 80% of patients with AIDS. Similarly, cultures of CSF are usually positive. Both the serum and the CSF cryptococcal antigen tests are accurate for the diagnosis of cryptococcal meningitis, with sensitivity and specificity greater than 90%.
The recommended treatment of cryptococcal meningitis has three stages, particularly in patients with HIV infection:95,96
Chronic suppression can be discontinued if patients remain asymptomatic and have a sustained increase in CD4 counts of more than 100 to 200/μL for at least 6 months after antiretroviral therapy.95
Patients without HIV infection either follow the same treatment algorithm as for HIV patients or are treated with a 6- to 10-week regimen of amphotericin B, with or without initial flucytosine.94 If the 6- to 10-week regimen is selected, the decision to discontinue treatment will depend on resolution of symptoms, negative CSF cultures on at least two occasions, and normal CSF values. Patients with ongoing immunosuppression that may have initially predisposed them to cryptococcal infection will require an additional 6 to 12 months of fluconazole therapy after these criteria are met. Increased intracranial pressure is a common feature of cryptococcal meningitis, particularly in patients with AIDS.97 It may result in a delayed clinical response and death; therefore, decompression may be required through drainage of the CSF by either serial lumbar punctures or placement of a shunt.
Central Nervous System Histoplasmosis
H. capsulatum is a dimorphic fungus, which means that it occurs as a mold (mycelial form) in nature and as a yeast in tissue (and at temperatures less than 37°C in cultures).98 The yeast form is a small, thin-walled, oval structure measuring 2 to 4mm in diameter. H. capsulatum is found in temperate zones around the world. In the United States, it is endemic in the Ohio and Mississippi river valleys.99 Its natural habitat is soil, especially soil with high organic content such as soil enriched with bird, chicken, or bat excrement.
Infection is acquired by inhalation and deposition of mycelial fragments into the alveoli. The organism converts to its pathogenic yeast form and is phagocytosed by alveolar macrophages. Specific T cell–mediated immunity develops and inhibits the growth of the organisms. However, the persistence of the organisms within granulomas can lead to reactivation or dissemination (or both) of the disease during the immunosuppressed state.100
CNS involvement occurs either as an isolated infection or as part of a progressive disseminated disease.101,102 In disseminated disease, CNS involvement is noted in 5% to 10% of cases. Clinical presentation varies widely and includes meningitis, diffuse encephalitis, focal neurological deficits, and stroke syndromes. Unless the yeast is identified in different organs, the diagnosis of CNS infection itself may be problematic.101–103 CSF findings are nonspecific, usually with lymphocytic pleocytosis, decreased glucose levels, and increased protein levels. CSF cultures are often negative early in the disease, and they require multiple specimens, large volumes (greater than 10 mL), and an extended incubation period (longer than 35 days). Serological tests for anti-Histoplasma antibodies in the CSF may be positive in up to 80% of cases. Testing for Histoplasma polysaccharide antigen in the CSF can be useful in 38% to 67% of cases.104
The treatment of choice for CNS histoplasmosis is amphotericin B, 0.7 to 1 mg/kg per day for a total dose of 30 to 35 mg/kg, followed by fluconazole, 800 mg daily for 9 to 12 months.96,105 Itraconazole does not penetrate the blood-brain barrier well and therefore should not be used. Criteria for discontinuing treatment include a normal concentration of CSF glucose and an undetectable level of CSF Histoplasma polysaccharide antigen. If immunodeficiency cannot be reversed, lifelong maintenance therapy may be required. Some clinicians prefer lipid formulations of amphotericin B because they deliver higher doses with less toxicity. If lipid formulations are used, they should be administered at dosages of 3 to 5 mg/kg per day for a total of 100 to 150 mg/kg over 6 to 12 weeks.
Central Nervous System Blastomycosis
B. dermatitidis is also a dimorphic fungus.98 The yeast cells are round and thick walled with daughter cells forming from a broad-based bud. It is an endemic fungus found primarily in the south central, southeastern, and midwestern United States, especially in the states along the Mississippi and Ohio rivers, as well as in the Canadian provinces bordering the Great Lakes.106 Occasionally, cases have also been reported in Africa, India, the Middle East, Central America, and South America. Outdoor activities near waterways and exposure to dust from construction and excavation sites are the major risk factors for acquiring infection.
Infection begins with inhalation of the organism, which then enters the lungs and converts to its yeast phase. Infection at this stage is usually asymptomatic. Cellular immunity is the major host protective factor in preventing progression of disease. Symptomatic disease develops in about one half of infected individuals. In some, the infection spreads via the bloodstream and lymphatics to distant sites, most commonly skin, bone, and the genitourinary system.107
CNS involvement is uncommon (less than 5%) in immune-competent patients with blastomycosis.108,109 In contrast, as many as 40% of AIDS patients with blastomycosis have been reported to have CNS disease, usually as a fulminant complication of widely disseminated blastomycosis.110 CNS blastomycosis manifests itself as either an abscess or meningitis. Both are difficult to diagnose in the absence of a diagnosis from another site in the body. CSF cultures are rarely positive, and culture of ventricular fluid may have a higher yield. Biopsy of cranial abscesses may be required for identification of the organism.
For patients with CNS blastomycosis, amphotericin B deoxycholate remains the drug of choice.111 A total dose of at least 2g is usually recommended. For patients unable to tolerate a full course of amphotericin B, fluconazole at a dosage of 800 mg/day can be substituted. Human immunodeficiency virus–infected or otherwise significantly immunocompromised patients with CNS blastomycosis require long-term suppressive therapy with fluconazole.96
Central Nervous System Coccidioidomycosis
C. immitis is a dimorphic fungus endemic to certain areas in the southwestern United States and parts of Mexico, Central America, and South America.112 The yeast form has a unique spherical structure known as a spherule, which is a large, round, thick-walled structure filled with daughter cells or endospores. As a spherule matures, its outer wall becomes thinner and eventually ruptures. This results in the release of endospores, which may propagate further in tissue.
Infection is acquired by inhalation of the organism and the subsequent formation of a lung lesion is the consequence of an inflammatory response. Many of the infections are either asymptomatic or mild and self-limited.113 As with the other endemic fungi, control of the infection is dependent on cell-mediated immunity.
Coccidioidomycosis rarely spreads beyond the lungs. However, immunodeficiency conditions, such as in human immunodeficiency virus infection, immunosuppressive therapy, Hodgkin lymphoma, and transplant recipients, dramatically increase the risk of dissemination.114,115 Other conditions that increase the likelihood of dissemination include pregnancy and African or Filipino ancestry. Up to one third of disseminated cases present with meningitis. Infection predominantly affects the basilar meninges. However, intracerebral abscesses occasionally develop.116,117 The most common symptoms are headache, nausea, vomiting, and altered mental status. Hydrocephalus is a common complication of coccidioidal meningitis.
CSF analysis shows mononuclear pleocytosis with decreased glucose and increased protein concentrations. CSF eosinophilia occurs in up to 70% of cases. Serological testing plays a more important role in the diagnosis and management of coccidioidomycosis than of other fungal infections.118 Tube precipitin-reacting antibodies disappear and are not found in chronic infections, whereas complement-fixing antibodies are present as long as infection persists; they disappear when the infection resolves. Complement-fixing antibodies can also be detected in the CSF of patients with meningitis and similarly can be useful for monitoring disease. Culture of the CSF is often negative.
The standard treatment for coccidioidal meningitis had been intrathecal amphotericin B, but this treatment is now reserved for patients who do not respond to oral azole therapy. The current preferred initial treatment for coccidioidal meningitis is oral fluconazole at 400 mg/day.119 Some patients may need higher doses of fluconazole. Treatment should be continued for at least 1 year and for 6 months after all evidence of further improvement has ceased. Relapses occur in approximately one third of patients when therapy is stopped, and some patients may require suppressive therapy indefinitely. In addition to antifungal therapy, a shunting procedure may be required to control hydrocephalus. If present, abscesses may require drainage or resection.
Central Nervous System Mucormycosis
The term mucormycosis describes a group of diseases caused by fungi that belong to the order Mucorales.120 The genera reported to cause invasive infection include Rhizopus, Absidia, Cunninghamella, Rhizomucor, Mucor, Apophysomyces, Saksenaea, Cokeromyces, and Syncephalastrum. These organisms are morphologically filamentous with broad, nonseptate hyphae with branches occurring at right angles. They are ubiquitous in nature and are common inhabitants of decaying matter and soil.
The most common clinical presentation of mucormycosis is rhinocerebral mucormycosis.121,122 As the name implies, infection starts in the nose and paranasal sinuses and spreads to the orbit and the adjacent structures of the brain, commonly the frontal lobe and the cavernous sinus. The clinical manifestations of rhinocerebral mucormycosis reflect the involvement of the tissues and structures. Initial complaints are facial pain and headache. Fever may not be present. With further progression of the infection, additional complaints and findings include erythema and ulceration of the palate, periorbital cellulitis, proptosis, swelling of the conjunctiva, cranial nerve dysfunction, loss of vision, mental status changes, and coma.
Isolated CNS mucormycosis has also been reported. The majority of these reports were in intravenous drug users.123 The most common clinical presentation includes mental status changes and focal neurological deficits. The infection appears to have a predilection for basal ganglia.
Treatment of mucormycosis needs to be prompt and aggressive. Both surgical débridement of necrotic tissue and antifungal therapy are required. Repeated débridement is often necessary. The agents of mucormycosis are relatively refractory to antifungal therapy. They require high doses of amphotericin B deoxycholate, typically 1.0 to 1.5 mg/kg per day. Lipid preparations of amphotericin B are acceptable alternatives and may permit the delivery of high doses of amphotericin B with less toxicity. None of the currently available azoles or echinocandins are active against the organisms that cause mucormycosis. However, posaconazole, a new azole that is currently in phase III trials and is also available in compassionate use protocols, has been reported to have good activity against some of the agents that cause mucormycosis.124,125 The overall mortality rate of mucormycosis is approximately 50%.
Central Nervous System Aspergillosis
Aspergillus is a mold that is ubiquitous in the environment worldwide, being found primarily in decaying vegetation but also in soil and water. Its hyphae (filaments) are thin and septated, branching off at acute angles. The most common species causing invasive infection include Aspergillus fumigatus, Aspergillus flavus, Aspergillus terreus, and Aspergillus niger.126 Infection is usually acquired by inhalation into the lungs. In persons without effective host defenses (e.g., neutropenic patients, patients receiving corticosteroid treatment, and transplant recipients), the inhaled organisms enlarge and germinate, invade tissue, and eventually cause disseminated infection. Tissue and vascular invasiveness is the hallmark of Aspergillus infections.127
The most common clinical manifestation of Aspergillus infection is invasive pulmonary infection. CNS aspergillosis may occur in up to 10% to 20% of all cases of invasive aspergillosis. Reports suggest that it is being encountered more frequently than in the past. Although it usually occurs as part of a disseminated infection with concomitant pulmonary infection, isolated CNS aspergillosis has been reported, particularly in intravenous drug users. Even though it has been reported among patients with HIV infection, CNS aspergillosis does not appear to be more common in HIV patients than in other immunocompromised patients. Its most common manifestation is a brain abscess causing headache, focal neurological signs, and altered mental status. The spectrum of CNS disease includes meningitis, hemorrhages, bland infarctions, myelitis, epidural abscesses, and mycotic aneurysms.128–134
The diagnosis of CNS aspergillosis requires the demonstration of the organism and its invasion in CNS tissue or isolation of the organism from a biopsy specimen or CSF. Blood cultures are rarely positive. Computed tomography or magnetic resonance imaging of the brain most commonly shows single or multiple ring-enhancing lesions with surrounding edema. Other radiographic appearances include hemorrhagic infarction pattern, parenchymal hemorrhage pattern, and diffuse necrotic encephalitis pattern.135 An enzyme immunoassay for detecting Aspergillus galactomannan, an Aspergillus antigen, has been licensed in the United States for the diagnosis of invasive aspergillosis.136 Although not extensively evaluated, at least one report suggests that detection of Aspergillus galactomannan in CSF may be of diagnostic value.137
Historically, invasive aspergillosis as a whole is refractory to treatment, with overall mortality rates of 50% or more. CNS aspergillosis carries the poorest prognosis, with mortality rates of more than 90% reported in most series.138 The following antifungal agents are active against Aspergillus species: amphotericin B deoxycholate, lipid forms of amphotericin B, itraconazole, voriconazole, and caspofungin. Voriconazole is considered by many as the agent of first choice for the treatment of invasive aspergillosis. This may also apply for the treatment of CNS aspergillosis. New drugs in development, such as posaconazole, ravuconazole, micafungin, and anidulafungin are also active against Aspergillus species and may offer new options for therapy. All drugs need to be given at high doses, and the optimal duration of therapy is unknown. Although it would be reasonable to continue to treat until clinical and radiographic abnormalities have resolved, it may be necessary to continue treatment as long as the underlying predisposition for invasive Aspergillus infection exists. Stereotactic drainage of abscesses may be required.139
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