PARASITIC AND FUNGAL INFECTIONS

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CHAPTER 94 PARASITIC AND FUNGAL INFECTIONS

PARASITIC INFECTIONS OF THE CENTRAL NERVOUS SYSTEM

In 1909 John D. Rockefeller declared, “Deprivation in the agriculturally rich Southern States is not due to stupidity or laziness, but to parasite infestation.”1 The human immunodeficiency virus pandemic and global warming have resurrected the study of parasitology. In general, parasitic diseases are treatable and should be considered when geography, patient susceptibility, and exposure make infection possible.

More than 2 million people die each year of falciparum malaria; 200 million are infected with schistosomiasis. Toxoplasma species flourish in patients with acquired immunodeficiency syndrome (AIDS). Epilepsy from neurocysticercosis affects more than 1% of the population in some regions. Most cases of neurocysticercosis in Mexico remain undiagnosed despite the people having cognitive and psychiatric problems throughout life. Sleeping sickness affects 300,000 Africans and threatens many more. Intestinal helminths dull children’s minds.2

This chapter addresses the major neuroparasites, as is customary in the neurosciences, by location and clinical presentation. The aim is to present the clinician with a catalog of possibilities so that a treatable disease is not overlooked (Table 94-1).

TABLE 94-1 Clinical Findings Associated with Neuroparasites

CSF, cerebrospinal fluid; MRI, magnetic resonance imaging.

The pathophysiology of parasites illustrates how they have adapted to evade the host’s immune system through pleomorphism and antigenic variation (malaria and sleeping sickness) or how they avoid killing their host to ensure their survival (hookworms). Parasites stimulate the secretion of prostaglandins, nitric oxide, and interleukin-10, which downregulate the immune response (Plasmodium, Trypanosoma, and Toxoplasma), ensuring their quiet multiplication and perhaps teaching us how to find and eliminate them when they cause disease.

Amebic Encephalitis

Various features of primary amebic meningoencephalitis (a fulminant encephalitis) and granulomatous amebic encephalitis (a subacute granulomatous disease) are summarized in Table 94-2.

TABLE 94-2 Central Nervous System Amebiasis

Feature PAM GAE
Risk factors Swimming Diabetes, pregnancy, alcohol/cirrhosis, corticosteroids, AIDS, chemotherapy/radiotherapy
Organism Naegleria Acanthamoeba27,28
Balamuthia
Route to CNS Olfactory epithelium/nerves Intranasal/intracranial vasculitis leading to thrombosis and infarction
CNS disease Culture-negative, fulminant, purulent meningitis; mostly PMN, unlike TB or viral Focal or diffuse encephalitis with meningism, giant cell reaction29
Organism can harbor Yes30 Yes
Legionella, Vibrio cholerae
CSF laboratory findings Glucose variable; protein >1 g/L Glucose variable
CSF microscopic findings Motile Naegleria move 1-3 body lengths/min11 Lymphocytic pleocytosis; trophozoite seldom found in CSF31,32

AIDS, acquired immunodeficiency syndrome; CNS, central nervous system; CSF, cerebrospinal fluid; GAE, granulomatous amebic encephalitis; PAM, primary amebic meningoencephalitis; PMN, polymorphonuclear neutrophils; TB, tuberculosis.

African Trypanosomiasis

Clinical Presentation

A painful chancre is seen in approximately 50% of patients infected with T. b. rhodesiense but is seldom seen in patients infected with T. b. gambiense. The chancre is more common in non-Africans. It develops 5 to 15 days after the bite and heals in 3 to 4 weeks. It occasionally ulcerates and may be associated with regional lymphadenopathy.

The first stage of illness is associated with intermittent fever, headache, intense pruritus, facial edema, ocular symptoms, and arthralgia. Posterior cervical lymphadenopathy (Winterbottom’s sign) is prominent in T. b. gambiense infections. This is followed by an asymptomatic phase for several months or years.

The second stage, the meningoencephalitic stage, occurs after months to years (earlier with T. b. rhodesiense infections) and is associated with various neurological abnormalities. The parasite is found in the basal ganglia of laboratory animals, which is confirmed with magnetic resonance imaging in case reports. Fasciculations, tremor, rigidity, chorea, and ataxia are observed. Typically, the patient complains of severe frontal head pain, tremors, delayed hyperesthesia (Kerandel sign, which is pain elicited by a tap on a tendon and is common in Caucasians), pronounced lethargy, and day-night inversion (sleeping sickness). Cognitive decline is common, with memory loss, dementia, depression, agitation, and hallucinations. In addition, T. b. rhodesiense can cause myocarditis, pericardial effusion, and heart failure.

Treatment

If CSF is positive for leukocytes and IgM concentrations are high, melarsoprol (a trivalent arsenic compound) is the drug of choice (Table 94-3). Relapse rates are high, and up to 20% of patients infected with T. b. gambiense do not respond to melarsoprol. Eflornithine is an alternative therapy for infections from T. b. gambiense but not from T. b. rhodesiense. Melarsoprol may cause post-treatment reactive encephalopathy in up to 10% of cases, with a mortality rate of 50%. Thus, the overall mortality from melarsoprol is 5% from arsenic encephalopathy.

TABLE 94-3 Treatment of Trypanosoma brucei gambiense and Trypanosoma brucei rhodesiense Sleeping Sickness*

Stage West African (T. b. gambiense) East African (T. b. rhodesiense)
Early stage
Endemic countries According to national legislation or guidelines According to national legislation or guidelines
Other countries Pentamidine isethionate, 4 mg/kg body weight daily or on alternate days for 7 to 10 days intravenously or intramuscularly Suramin, test dose of 4-5 mg/kg body weight at day 1, followed by 5 injections of 20 mg/kg body weight every 7 days (e.g., days 3, 10, 17, 24, 31); the maximum dose per injection is 1 g
Late stage
Endemic countries According to national legislation or guidelines According to national legislation or guidelines
Other countries If available: eflornithine, 100 mg/kg body weight at 6-hour intervals for 14 days (150 mg/kg body weight in children) by short infusions over a period of at least 30 minutes Eflornithine not recommended (low efficacy38)
Alternative: melarsoprol, 3 series of 3.6 mg/kg body weight at 24-hour intervals for 3 days; the series are spaced by intervals of 7 days Melarsoprol,§ 1 series of 1.8, 2.16, 2.52 mg/kg body weight at 24-hour intervals; 1 series of 2.52, 2.88, 3.25 mg/kg body weight at 24-hour intervals; and 1 series of 3.6, 3.6, 3.6 mg/kg body weight at 24-hour intervals; the series are spaced by intervals of 7 days (the maximum dose is 5 mL)

* No firm recommendations exist for the use of the trypanocidal drugs; the schedules indicated are those most commonly used. A concise treatment schedule for treatment of T. b. gambiense sleeping sickness consisting of 10 days of melarsoprol, 2.2 mg/kg body weight daily, is under final evaluation.39,40 Note: This 10-day schedule must not be used for treatment of T. b. rhodesiense sleeping sickness, because there are no data available.

Very slow intravenous injection or short infusion, only under well-controlled circumstances.

The concomitant application of 1 mg/kg body weight prednisolone has been shown to reduce the incidence of encephalopathic syndromes in one large-scale clinical trial.41

§ A single dose of Suramin is often applied before the stage determining lumbar puncture.

From Burri C, Brun R: Human African trypanosomiasis. In Cook GC, Zumla AI, eds: Manson’s Tropical Diseases, 21st ed. Edinburgh: Saunders, 2003, pp 1303-1323. Used with permission.

A lumbar puncture should be performed 1 day after a course of therapy for late-stage disease. All patients should be monitored for 2 years with lumbar punctures every 6 months. A relapse is suspected if the CSF cell count is more than 20 cells/μL. Reinfection is likely when CSF has more than 50 cells/μL, when the count has doubled since the previous count, or when there are 20 to 49 cells/μL in a symptomatic patient.25,39

Cysticercus of Taenia solium

Diagnosis

On the basis of autopsy findings in Mexico, most cases of neurocysticercosis remain undiagnosed despite the patient having cognitive and psychiatric problems throughout life. Subcutaneous cysts are palpable. In striated muscle, they rapidly calcify and may be noted on imaging studies. Stool examination is unrewarding. Peripheral eosinophilia is inconsistent and nonspecific.

Blood and CSF serological analyses have improved. Many clinicians use the enzyme-linked immunoelectrotransfer blot (EITB) assay. It derives from coproantigens of adult T. solium and ignores nonspecific bands of antigen. EITB results may revert to negative after the cysticercus dies. The assay is not always readily available. In the blood, sensitivity is 92.5% and specificity is 100%. However, EITB sensitivity has been reported to be as low as 28% in subjects with a single parenchymal lesion, as is commonly seen in India.46 EITB sensitivity may be less in CSF.47

Enzyme-linked immunosorbent assay (ELISA) is based on crude antigen to cysticercal fluid. It is inexpensive and rapid. ELISA cross-reacts with other cestodes (other taeniae and echinococci). It seems more sensitive in CSF (sensitivity, 62% to 90%; specificity 98% to 100%) than in blood.

The diagnosis requires neuroimaging, with the goal of identifying the scolex (2 to 3 mm). Only since the frequent use of computed tomography in the 1980s has cysticercosis emerged as the most common cause of epilepsy in many areas. (Cerebral malaria is likely the most common cause of febrile seizures.) Magnetic resonance imaging may show a diagnostic invaginated scolex but not show the calcific stage, for which computed tomography is best. Magnetic resonance imaging can show ventricular cysts. In brain parenchyma, the cysts mature in 3 months to 10mm in size (occasionally up to 20mm); in the ventricles, they can exceed 5 cm. Therefore, serum EITB, CSF ELISA, and neuroimaging have become useful diagnostic tools48,49 (Figs. 94-1 through 94-7).

image

Figure 94-1 Inactive form (nodular stage) of cysticercosis. Axial computed tomography shows small calcification in the left occipital lobe.

(From Castillo M: Imaging of neurocysticercosis. Semin Roentgenol 2004; 39:465-473. Used with permission.)

Treatment

image

Figure 94-8 Antiepileptic treatment for patients with first seizure due to neurocysticercosis.52 Asterisk indicates that “degenerative” includes a single enhancing lesion on computed tomography (computed tomography) after differential diagnosis has been established. AEM, antiepileptic medication; MRI, magnetic resonance imaging.

(From Carpio A: Neurocysticercosis: an update. Lancet Infect Dis 2002; 2:751-762. Used with permission.)

Falciparum Malaria

Clinical Presentation

Severe falciparum malaria is clearly defined and should be treated parenterally initially (Table 94-5). Patients with cerebral malaria present with coma, convulsions, dysconjugate gaze, pouting, and bruxism. Retinal hemorrhages are seen. CNS findings are symmetrical. The plantar reflexes are upgoing.68

TABLE 94-5 Severe Falciparum Malaria

Modified from the World Health Organization: Management of Severe Malaria: A Practical Handbook, 2nd ed. Geneva: World Health Organization, 2000, p 5. Used with permission.

In children with febrile convulsions followed by coma, 30 to 60 minutes may have to pass before malaria is diagnosed, but treatment should not be delayed. Patients with malarial convulsions may present with only nystagmus, salivation, and twitching of the mouth or a digit. One third of seizures in children manifest only as eye deviation or salivation, or both. One in 10 children with severe malaria has sequelae: ataxia, hemiparesis (hemiconvulsion-hemiparesis syndrome), speech disorder, blindness, behavioral problems, cognitive difficulties (executive functions), and spasticity. Prolonged seizures, coma, hypoglycemia, and absence of hyperpyrexia are associated with neurological sequelae.69

Hypoglycemia not accompanied by sweating occurs in 20% of children and pregnant women but also in 8% of adults with severe malaria. It is caused by malaria itself (the parasite consumes glucose) or quinine-induced hyperinsulinemia. Hypoglycemia also results in coma, convulsions, extensor posturing, and shock. Acidosis and Kussmaul respiration are common. Respiratory distress is poorly understood and is the main cause of death in African children. Brainstem disturbance has been implicated.

Treatment

Common errors in treating malaria are the following:

Treatment guidelines with quinine and quinidine for severe malaria are as follows:

The artemisinins clear parasitemia faster than quinine:

Corticosteroids are associated with an increased risk of adverse side effects and do not decrease mortality. The diagnosis and management of severe malaria70 and therapeutic options are discussed in other publications.25,7076

Paragonimus

Clinical Presentation

The lung infection can be mistaken for bronchiectasis. It may be misdiagnosed as nocardiosis, tuberculosis, or metastatic lung cancer. Symptoms may be recognized for years before diagnosis.79 Epilepsy occurs in 65% to 95% of CNS cases before diagnosis. Chronic mild insidious eosinophilic meningitis (the majority of cases), or recurrent acute meningitis, as well as chronic decline in mental status persisting for as long as 20 years is described. Homonymous hemianopia is common.80,81 Flukes in the spinal cord cause spastic paraplegia.82

Diagnosis

Chest radiographic abnormalities are seen in 80% of patients. Oh83 reviewed 62 cases of cerebral paragonimiasis and found CSF eosinophilia in 8% and peripheral eosinophilia in 41%. If available, serology by enzyme-linked immunosorbent assay and complement fixation on CSF samples may be used for diagnosis and for monitoring therapy. Microscopic examination is performed on stool, gastric washings, sputum, or tissue.84

In chronic brain syndrome, magnetic resonance imaging shows multiple conglomerated, round, enhancing nodules (“soap bubbles”) with encephalomalacia in the temporal, parietal, and occipital lobes. Computed tomography scans show large calcified nodules or multiple cystic calcifications in the same region in 40% to 70% of cases.20,80,82,83

Schistosoma japonicum

Schistosoma mansoni and Schistosoma haematobium

Toxoplasma gondii

Diagnosis

Toxoplasmosis can be diagnosed in several ways: isolation of the organism from the placenta in neonates, mouse inoculation or inoculation of tissue cell cultures; amplification of its DNA in blood or body fluids; demonstration of the organism in histological sections of tissue or in cytological preparations of body fluids; and characteristic serological test results.

Serology has been the primary method of diagnosis of toxoplasmosis. IgG antibodies usually appear within 1 to 2 weeks after the infection is acquired, reach peak concentrations within 1 to 2 months, and usually persist for life. IgM antibodies may appear earlier and decline more rapidly than IgG antibodies, but they also can persist for years. CNS toxoplasmosis in general is a reactivation disease; therefore, the usefulness of serological testing is primarily to determine whether the patient has been previously exposed to the parasite. However, demonstration of intrathecal production of IgG or IgM antibodies against T. gondii is diagnostic of toxoplasma encephalitis. In patients with AIDS, the presence of toxoplasma IgG in association with multiple intracranial lesions, in the absence of prophylaxis against Toxoplasma, has a positive predictive value of approximately 80% for toxoplasma encephalitis and thus mandates empirical antitoxoplasmosis therapy.

Polymerase chain reaction has been used successfully on samples of CSF and brain tissue. The sensitivity of polymerase chain reaction in CSF varies between 11% and 77%; the specificity approaches 100%. Therapy for toxoplasmosis decreases the sensitivity of polymerase chain reaction; sensitivity is higher in CSF or blood samples collected before or within the first week of therapy.

FUNGAL INFECTIONS OF THE CENTRAL NERVOUS SYSTEM

Although many fungal species can infect the CNS, most fungal CNS infections are caused by a relatively few important fungal organisms. In general, CNS infections caused by fungal organisms manifest either as meningitis or space-occupying lesions (including abscesses), or both. The following discussion describes the general characteristics of CNS infections caused by some of the leading fungal organisms, including Cryptococcus neoformans, Histoplasma capsulatum, Blastomyces dermatitidis, Coccidioides immitis, agents of mucormycosis, and Aspergillus species.

Central Nervous System Cryptococcosis

C. neoformans is a round or oval yeast that has a worldwide distribution and is found particularly in bird excreta.91 Infection is acquired through inhalation of the organism. Most pulmonary infections are asymptomatic, but acute symptomatic pneumonia may occur. Infection can disseminate to distant sites either during this primary lung infection or later during reactivation, usually as a result of cell-mediated immune deficiency.92 The most likely site for dissemination is the CNS, but the reason is not entirely clear. Some investigators have hypothesized that there is a receptor in the CNS that binds a ligand on the organism, but this hypothesis has not been verified.

Patients with CNS cryptococcosis usually present with a subacute meningitis, with signs and symptoms such as headache, fever, and focal neurological deficits occurring over several weeks.93,94 Symptoms may be subtle, waxing and waning. Classic meningeal symptoms (e.g., neck stiffness or photophobia) may not always be present. In particular, in human immunodeficiency virus infection, they are present in only one fourth to one third of patients. Brain imaging studies are also nonspecific; computed tomography scans may be normal in one half the cases. Cerebral atrophy and ventricular enlargement are the most common findings in human immunodeficiency virus–infected patients. Cryptococcomas (nodules in the brain parenchyma) can also be present occasionally, especially in patients with infection caused by C. neoformans var. gattii. CSF findings usually include an increased opening pressure, mild mononuclear pleocytosis, and increased protein concentration. India ink examination of the CSF is positive in 50% of non-AIDS patients with cryptococcal meningitis and in more than 80% of patients with AIDS. Similarly, cultures of CSF are usually positive. Both the serum and the CSF cryptococcal antigen tests are accurate for the diagnosis of cryptococcal meningitis, with sensitivity and specificity greater than 90%.

The recommended treatment of cryptococcal meningitis has three stages, particularly in patients with HIV infection:95,96

Chronic suppression can be discontinued if patients remain asymptomatic and have a sustained increase in CD4 counts of more than 100 to 200/μL for at least 6 months after antiretroviral therapy.95

Patients without HIV infection either follow the same treatment algorithm as for HIV patients or are treated with a 6- to 10-week regimen of amphotericin B, with or without initial flucytosine.94 If the 6- to 10-week regimen is selected, the decision to discontinue treatment will depend on resolution of symptoms, negative CSF cultures on at least two occasions, and normal CSF values. Patients with ongoing immunosuppression that may have initially predisposed them to cryptococcal infection will require an additional 6 to 12 months of fluconazole therapy after these criteria are met. Increased intracranial pressure is a common feature of cryptococcal meningitis, particularly in patients with AIDS.97 It may result in a delayed clinical response and death; therefore, decompression may be required through drainage of the CSF by either serial lumbar punctures or placement of a shunt.

Central Nervous System Histoplasmosis

H. capsulatum is a dimorphic fungus, which means that it occurs as a mold (mycelial form) in nature and as a yeast in tissue (and at temperatures less than 37°C in cultures).98 The yeast form is a small, thin-walled, oval structure measuring 2 to 4mm in diameter. H. capsulatum is found in temperate zones around the world. In the United States, it is endemic in the Ohio and Mississippi river valleys.99 Its natural habitat is soil, especially soil with high organic content such as soil enriched with bird, chicken, or bat excrement.

Infection is acquired by inhalation and deposition of mycelial fragments into the alveoli. The organism converts to its pathogenic yeast form and is phagocytosed by alveolar macrophages. Specific T cell–mediated immunity develops and inhibits the growth of the organisms. However, the persistence of the organisms within granulomas can lead to reactivation or dissemination (or both) of the disease during the immunosuppressed state.100

CNS involvement occurs either as an isolated infection or as part of a progressive disseminated disease.101,102 In disseminated disease, CNS involvement is noted in 5% to 10% of cases. Clinical presentation varies widely and includes meningitis, diffuse encephalitis, focal neurological deficits, and stroke syndromes. Unless the yeast is identified in different organs, the diagnosis of CNS infection itself may be problematic.101103 CSF findings are nonspecific, usually with lymphocytic pleocytosis, decreased glucose levels, and increased protein levels. CSF cultures are often negative early in the disease, and they require multiple specimens, large volumes (greater than 10 mL), and an extended incubation period (longer than 35 days). Serological tests for anti-Histoplasma antibodies in the CSF may be positive in up to 80% of cases. Testing for Histoplasma polysaccharide antigen in the CSF can be useful in 38% to 67% of cases.104

The treatment of choice for CNS histoplasmosis is amphotericin B, 0.7 to 1 mg/kg per day for a total dose of 30 to 35 mg/kg, followed by fluconazole, 800 mg daily for 9 to 12 months.96,105 Itraconazole does not penetrate the blood-brain barrier well and therefore should not be used. Criteria for discontinuing treatment include a normal concentration of CSF glucose and an undetectable level of CSF Histoplasma polysaccharide antigen. If immunodeficiency cannot be reversed, lifelong maintenance therapy may be required. Some clinicians prefer lipid formulations of amphotericin B because they deliver higher doses with less toxicity. If lipid formulations are used, they should be administered at dosages of 3 to 5 mg/kg per day for a total of 100 to 150 mg/kg over 6 to 12 weeks.

Central Nervous System Blastomycosis

B. dermatitidis is also a dimorphic fungus.98 The yeast cells are round and thick walled with daughter cells forming from a broad-based bud. It is an endemic fungus found primarily in the south central, southeastern, and midwestern United States, especially in the states along the Mississippi and Ohio rivers, as well as in the Canadian provinces bordering the Great Lakes.106 Occasionally, cases have also been reported in Africa, India, the Middle East, Central America, and South America. Outdoor activities near waterways and exposure to dust from construction and excavation sites are the major risk factors for acquiring infection.

Infection begins with inhalation of the organism, which then enters the lungs and converts to its yeast phase. Infection at this stage is usually asymptomatic. Cellular immunity is the major host protective factor in preventing progression of disease. Symptomatic disease develops in about one half of infected individuals. In some, the infection spreads via the bloodstream and lymphatics to distant sites, most commonly skin, bone, and the genitourinary system.107

CNS involvement is uncommon (less than 5%) in immune-competent patients with blastomycosis.108,109 In contrast, as many as 40% of AIDS patients with blastomycosis have been reported to have CNS disease, usually as a fulminant complication of widely disseminated blastomycosis.110 CNS blastomycosis manifests itself as either an abscess or meningitis. Both are difficult to diagnose in the absence of a diagnosis from another site in the body. CSF cultures are rarely positive, and culture of ventricular fluid may have a higher yield. Biopsy of cranial abscesses may be required for identification of the organism.

For patients with CNS blastomycosis, amphotericin B deoxycholate remains the drug of choice.111 A total dose of at least 2g is usually recommended. For patients unable to tolerate a full course of amphotericin B, fluconazole at a dosage of 800 mg/day can be substituted. Human immunodeficiency virus–infected or otherwise significantly immunocompromised patients with CNS blastomycosis require long-term suppressive therapy with fluconazole.96

Central Nervous System Coccidioidomycosis

C. immitis is a dimorphic fungus endemic to certain areas in the southwestern United States and parts of Mexico, Central America, and South America.112 The yeast form has a unique spherical structure known as a spherule, which is a large, round, thick-walled structure filled with daughter cells or endospores. As a spherule matures, its outer wall becomes thinner and eventually ruptures. This results in the release of endospores, which may propagate further in tissue.

Infection is acquired by inhalation of the organism and the subsequent formation of a lung lesion is the consequence of an inflammatory response. Many of the infections are either asymptomatic or mild and self-limited.113 As with the other endemic fungi, control of the infection is dependent on cell-mediated immunity.

Coccidioidomycosis rarely spreads beyond the lungs. However, immunodeficiency conditions, such as in human immunodeficiency virus infection, immunosuppressive therapy, Hodgkin lymphoma, and transplant recipients, dramatically increase the risk of dissemination.114,115 Other conditions that increase the likelihood of dissemination include pregnancy and African or Filipino ancestry. Up to one third of disseminated cases present with meningitis. Infection predominantly affects the basilar meninges. However, intracerebral abscesses occasionally develop.116,117 The most common symptoms are headache, nausea, vomiting, and altered mental status. Hydrocephalus is a common complication of coccidioidal meningitis.

CSF analysis shows mononuclear pleocytosis with decreased glucose and increased protein concentrations. CSF eosinophilia occurs in up to 70% of cases. Serological testing plays a more important role in the diagnosis and management of coccidioidomycosis than of other fungal infections.118 Tube precipitin-reacting antibodies disappear and are not found in chronic infections, whereas complement-fixing antibodies are present as long as infection persists; they disappear when the infection resolves. Complement-fixing antibodies can also be detected in the CSF of patients with meningitis and similarly can be useful for monitoring disease. Culture of the CSF is often negative.

The standard treatment for coccidioidal meningitis had been intrathecal amphotericin B, but this treatment is now reserved for patients who do not respond to oral azole therapy. The current preferred initial treatment for coccidioidal meningitis is oral fluconazole at 400 mg/day.119 Some patients may need higher doses of fluconazole. Treatment should be continued for at least 1 year and for 6 months after all evidence of further improvement has ceased. Relapses occur in approximately one third of patients when therapy is stopped, and some patients may require suppressive therapy indefinitely. In addition to antifungal therapy, a shunting procedure may be required to control hydrocephalus. If present, abscesses may require drainage or resection.

Central Nervous System Mucormycosis

The term mucormycosis describes a group of diseases caused by fungi that belong to the order Mucorales.120 The genera reported to cause invasive infection include Rhizopus, Absidia, Cunninghamella, Rhizomucor, Mucor, Apophysomyces, Saksenaea, Cokeromyces, and Syncephalastrum. These organisms are morphologically filamentous with broad, nonseptate hyphae with branches occurring at right angles. They are ubiquitous in nature and are common inhabitants of decaying matter and soil.

Inhalation is the natural route of infection. However, traumatic inoculation has also been described. Undefined defects in immunity in immunocompromised patients and in diabetic patients permit the proliferation of the spores. The hallmark of mucormycosis is the direct invasion of blood vessels by these organisms and the subsequent thrombosis and tissue necrosis.

The most common clinical presentation of mucormycosis is rhinocerebral mucormycosis.121,122 As the name implies, infection starts in the nose and paranasal sinuses and spreads to the orbit and the adjacent structures of the brain, commonly the frontal lobe and the cavernous sinus. The clinical manifestations of rhinocerebral mucormycosis reflect the involvement of the tissues and structures. Initial complaints are facial pain and headache. Fever may not be present. With further progression of the infection, additional complaints and findings include erythema and ulceration of the palate, periorbital cellulitis, proptosis, swelling of the conjunctiva, cranial nerve dysfunction, loss of vision, mental status changes, and coma.

Isolated CNS mucormycosis has also been reported. The majority of these reports were in intravenous drug users.123 The most common clinical presentation includes mental status changes and focal neurological deficits. The infection appears to have a predilection for basal ganglia.

The presence of tissue necrosis (e.g., black eschars and discharges) is suggestive of the disease; attempts at diagnosis and treatment should be prompt and aggressive. The diagnosis of mucormycosis requires the identification of the organisms in tissue. The agents of mucormycosis are usually difficult to grow in culture and blood cultures are rarely positive. Imaging studies (computed tomography and magnetic resonance imaging) of the sinuses and the brain are helpful in establishing the extent of involvement.

Treatment of mucormycosis needs to be prompt and aggressive. Both surgical débridement of necrotic tissue and antifungal therapy are required. Repeated débridement is often necessary. The agents of mucormycosis are relatively refractory to antifungal therapy. They require high doses of amphotericin B deoxycholate, typically 1.0 to 1.5 mg/kg per day. Lipid preparations of amphotericin B are acceptable alternatives and may permit the delivery of high doses of amphotericin B with less toxicity. None of the currently available azoles or echinocandins are active against the organisms that cause mucormycosis. However, posaconazole, a new azole that is currently in phase III trials and is also available in compassionate use protocols, has been reported to have good activity against some of the agents that cause mucormycosis.124,125 The overall mortality rate of mucormycosis is approximately 50%.

Central Nervous System Aspergillosis

Aspergillus is a mold that is ubiquitous in the environment worldwide, being found primarily in decaying vegetation but also in soil and water. Its hyphae (filaments) are thin and septated, branching off at acute angles. The most common species causing invasive infection include Aspergillus fumigatus, Aspergillus flavus, Aspergillus terreus, and Aspergillus niger.126 Infection is usually acquired by inhalation into the lungs. In persons without effective host defenses (e.g., neutropenic patients, patients receiving corticosteroid treatment, and transplant recipients), the inhaled organisms enlarge and germinate, invade tissue, and eventually cause disseminated infection. Tissue and vascular invasiveness is the hallmark of Aspergillus infections.127

The most common clinical manifestation of Aspergillus infection is invasive pulmonary infection. CNS aspergillosis may occur in up to 10% to 20% of all cases of invasive aspergillosis. Reports suggest that it is being encountered more frequently than in the past. Although it usually occurs as part of a disseminated infection with concomitant pulmonary infection, isolated CNS aspergillosis has been reported, particularly in intravenous drug users. Even though it has been reported among patients with HIV infection, CNS aspergillosis does not appear to be more common in HIV patients than in other immunocompromised patients. Its most common manifestation is a brain abscess causing headache, focal neurological signs, and altered mental status. The spectrum of CNS disease includes meningitis, hemorrhages, bland infarctions, myelitis, epidural abscesses, and mycotic aneurysms.128134

The diagnosis of CNS aspergillosis requires the demonstration of the organism and its invasion in CNS tissue or isolation of the organism from a biopsy specimen or CSF. Blood cultures are rarely positive. Computed tomography or magnetic resonance imaging of the brain most commonly shows single or multiple ring-enhancing lesions with surrounding edema. Other radiographic appearances include hemorrhagic infarction pattern, parenchymal hemorrhage pattern, and diffuse necrotic encephalitis pattern.135 An enzyme immunoassay for detecting Aspergillus galactomannan, an Aspergillus antigen, has been licensed in the United States for the diagnosis of invasive aspergillosis.136 Although not extensively evaluated, at least one report suggests that detection of Aspergillus galactomannan in CSF may be of diagnostic value.137

Historically, invasive aspergillosis as a whole is refractory to treatment, with overall mortality rates of 50% or more. CNS aspergillosis carries the poorest prognosis, with mortality rates of more than 90% reported in most series.138 The following antifungal agents are active against Aspergillus species: amphotericin B deoxycholate, lipid forms of amphotericin B, itraconazole, voriconazole, and caspofungin. Voriconazole is considered by many as the agent of first choice for the treatment of invasive aspergillosis. This may also apply for the treatment of CNS aspergillosis. New drugs in development, such as posaconazole, ravuconazole, micafungin, and anidulafungin are also active against Aspergillus species and may offer new options for therapy. All drugs need to be given at high doses, and the optimal duration of therapy is unknown. Although it would be reasonable to continue to treat until clinical and radiographic abnormalities have resolved, it may be necessary to continue treatment as long as the underlying predisposition for invasive Aspergillus infection exists. Stereotactic drainage of abscesses may be required.139

Suggested Reading

Benson CA, Kaplan JE, Masur H, et al. Treating opportunistic infections among HIV-infected adults and adolescents: recommendations from CDC, the National Institutes of Health, and the HIV Medicine Association/Infectious Diseases Society of America. Clin Infect Dis. 2005;40:S131-S235.

CDC Division of Parasitic Diseases. Parasitic disease information. Available at http://www.cdc.gov/ncidod/dpd/parasites/listing.htm.

Cook GC, Zumla AI, editors. Manson’s Tropical Diseases. Edinburgh: Saunders, 2003.

Cortez KJ, Walsh TJ. Space-occupying fungal lesions. In: Scheld WM, Whitley RJ, Marra CM, editors. Infections of the Central Nervous System. 3rd ed. Philadelphia: Lippincott Williams & Wilkins; 2004:713-734.

Drugs for parasitic infections. Med Lett Drugs Ther. Available at http://www.medletter.com/freedocs/parasitic.pdf, 2004.

Garcia HH, Del Brutto OH, Nash TE, et al. New concepts in the diagnosis and management of neurocysticercosis (Taenia solium). Am J Trop Med Hyg. 2005;72:3-9.

Perfect JR. Fungal meningitis. In: Scheld WM, Whitley RJ, Marra CM, editors. Infections of the Central Nervous System. 3rd ed. Philadelphia: Lippincott Williams & Wilkins; 2004:691-712.

Scheld WM, Whitley RJ, Marra CM, editors. Infections of the central nervous system, 3rd ed., Philadelphia: Lippincott Williams & Wilkins, 2004.

Sobel JD, Mycoses Study Group. Guidelines from the Infectious Diseases Society of America: practice guidelines for the treatment of fungal infections. Clin Infect Dis. 2000;30:652.

Walker M, Zunt JR. Parasitic central nervous system infections in immunocompromised hosts. Clin Infect Dis. 2005;40:1005-1015.

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