Parenchymal Liver Disease

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Chapter 90

Parenchymal Liver Disease

Hepatic Steatosis

Overview: Hepatic steatosis (fatty liver) is the most common cause of chronic liver disease in pediatric patients. It can range from simple steatosis to nonalcoholic steatohepatitis, which can progress to cirrhosis. Obesity and insulin resistance are the most common risk factors for hepatic steatosis, but it also may be associated with a number of metabolic processes and toxins (Box 90-1). The incidence is 2.6% in the general population, with a higher percentage in obese children.1 Adolescents are affected more often than younger children, with a higher prevalence in boys than girls. Fatty replacement of the liver may be diffuse or localized with areas of fatty sparing.

Clinical Presentation: Hepatic steatosis often is asymptomatic and is found incidentally on imaging performed for other reasons, such as nonspecific abdominal pain. Laboratory studies may show elevated liver enzymes (i.e., alanine aminotransferase and aspartate aminotransferase).1 Histologically, hepatocytes contain large cytoplasmic fat vacuoles filled with triglycerides. Pathologic diagnosis of hepatic steatosis is made when more than 5% of the total liver weight is replaced by fat.2

Imaging: Ultrasound evidence of hepatic steatosis includes variable hepatomegaly, increased echogenicity of the liver parenchyma when compared with the adjacent kidney, and poor visualization of the intrahepatic vascular structures (Fig. 90-1).2 Areas of fatty sparing are seen as hypoechoic foci within the fatty liver without mass effect and should not be mistaken for a mass. Ultrasound sensitivity for steatosis decreases with lower degrees of fatty infiltration. Increased obesity of the patient limits ultrasound quality because the increased extrahepatic fat further attenuates the ultrasound beam and decreases ultrasound sensitivity for liver abnormalities, including steatosis.

On computed tomography (CT), liver minus spleen attenuation of less than 1 Hounsfield unit (HU) can be used to screen for mild hepatic steatosis.3,4 Moderate to severe cases are indicated by liver minus spleen attenuation of less than −10 HU or absolute hepatic parenchymal attenuation less than 40 HU on noncontrast CT.4

Conventional spin-echo magnetic resonance imaging (MRI) is less sensitive than CT and ultrasound for detection of steatosis. Imaging findings include increased signal intensity on T1-weighted images and decreased signal intensity on fat-saturated and short tau inversion recovery sequences. However, chemical shift imaging with in-phase and out-of-phase sequences that demonstrates loss of signal on out-of-phase images is highly specific for diagnosing hepatic steatosis.2 This modality can be particularly helpful in differentiating this condition from neoplastic disease in equivocal cases, particularly in cases with focal fatty infiltration or sparing.

MR spectroscopy is a more recently described technique that may prove to be the most accurate method to quantitatively assess hepatic steatosis noninvasively. The area under the water and lipid peaks can be measured, allowing estimation of the hepatic fat fraction with a diagnostic accuracy of 80% to 85% in adult studies.2,5,6

The gold standard for diagnosis is biopsy and histologic evaluation. However, this approach is not without risk and is impractical in most pediatric patients with suspected hepatic steatosis.

Iron Deposition in the Liver

Overview: Approximately 80% of the normal iron stores of 2 to 6 g are in the form of hemoglobin, myoglobin, and enzymes that contain iron, and 20% are in the storage form of ferritin and hemosiderin. In normal situations, trace levels of iron are found in the liver, spleen, and bone marrow. When excess iron is present in the body, deposition may occur in the liver, spleen, lymph nodes, pancreas, kidneys, pituitary, and gastrointestinal (GI) tract. The body can compensate for some excess iron (10 to 20 g) without the occurrence of tissue damage, in which case the term hemosiderosis is applied. However, if functional and structural impairment of organs occurs as a result of excess iron (50 to 60 g), the term hemochromatosis is applied.

Two forms of hemochromatosis exist: primary and secondary. The primary form is the result of a genetic disorder that causes excess iron absorption through the gastrointestinal tract. This iron becomes bound to transferrin and eventually is stored as crystalline iron oxide within the cytoplasm of periportal hepatocytes. With progressive disease, the pancreas, synovium, heart, pituitary, and thyroid may be involved, but the Kupffer cells and reticuloendothelial cells of the bone marrow and spleen are spared.

The secondary, nongenetic form of hemochromatosis is more common than primary disease and may be the result of a myelodysplastic syndrome, anemia as a result of ineffective erythropoiesis, or exogenous sources from multiple transfusions, parenteral iron infusion, or ingestion. With secondary hemochromatosis, phagocytosis of intact red blood cells causes initial iron deposition within the reticuloendothelial system (i.e., the liver, spleen, and bone marrow). Once the storage capacity of the reticuloendothelial system is saturated, iron may accumulate within parenchymal cells of the organs, including the liver hepatocytes, pancreas, and myocardium, in a pattern similar to that of primary hemochromatosis.

Imaging: Ultrasound findings are nonspecific and noncontributory to the diagnosis of hemochromatosis. CT has a low sensitivity (63%) but high specificity (96%) for the diagnosis of iron overload.8 Noncontrast CT shows homogeneous increased density of the liver greater than 72 HU.8 However, coincident steatosis can lower the HU number, causing false-negative examinations. False-positive results may be seen with Wilson disease, gold therapy, or long-standing amiodarone treatment.

MR is the imaging modality of choice for confirming the diagnosis, determining severity, and monitoring therapy in persons with hemochromatosis. Excess iron deposition causes a proportional decrease in signal intensity on T1- and T2-weighted sequences and is most pronounced on gradient echo sequences. Skeletal muscle is unaffected by hemochromatosis and serves as a good internal reference to compare the signal intensity of affected abdominal organs and bone marrow, which will be hypointense to skeletal muscle. On in-phase and out-of-phase imaging, decreased signal intensity is seen in the affected organs on the in-phase sequence (an opposite finding from steatosis). The key findings in primary hemochromatosis are low signal intensity within the liver and pancreas on T2-weighted images (Fig. 90-2). In contradistinction, secondary hemochromatosis demonstrates a low signal in the liver, spleen, and bone marrow (Fig. 90-3) with sparing of the pancreas.9

MR quantification of liver iron concentration (LIC) is possible, eliminating the need for multiple biopsies to monitor hemochromatosis. One proposed method uses multiple gradient echo sequences (T1, PD, T2, and T2*). Three regions of interest are placed on the right lobe of the liver and two on skeletal muscle; these values then can be placed in an online algorithm that estimates LIC.10 A more recently described technique utilizes breath-hold multiecho T2*-weighted sequences and generation of a line plotting the natural log of hepatic signal intensity versus time to echo (TE). The slope of the line is R2* where 1/R2* = T2*. The T2* values then can be used to stratify different grades of LIC.11

Treatment: Treatment of hemochromatosis consists of scheduled phlebotomy or chelation therapy to reduce iron levels.7 As mentioned earlier, MR quantification of LIC may be used to follow treatment regimes, obviating the need for liver biopsies.

Glycogen Storage Diseases

Imaging: Sonography shows hepatomegaly with diffuse hepatic hyperechogenicity because of the combination of fatty replacement and glycogen deposition (e-Fig. 90-4).14,15 Superimposed hepatic adenomas are common. They are seen as well-defined masses with variable echogenicity (depending on the relative change in liver echotexture) and often demonstrate increased sound transmission and refractory shadowing at the margins. Because hepatic attenuation is increased by glycogen but decreased by fat, the CT findings are variable depending on which factor predominates. When fatty replacement predominates, the result is diffusely low attenuation of the liver.16 Hepatic adenomas likewise vary in appearance depending on the status of the liver; they appear hypodense when found in livers of normal attenuation (e-Fig. 90-5) but are variably hyperdense in the setting of a hepatic steatosis.

Gaucher Disease

Clinical Presentation: Three forms of Gaucher disease exist. Type 1 is the chronic nonneuropathic form and may present in childhood but commonly is recognized in the third to fourth decade. Type 2, the acute neuropathic or infantile form, rapidly leads to death and presents with severe hepatosplenomegaly, progressive seizures, mental retardation, spasticity, strabismus, and, rarely, skeletal manifestations. Type 3, the subacute neuropathic or juvenile form, is the rarest and presents between 2 and 6 years of age with hepatosplenomegaly, mild neurological symptoms, and late-onset skeletal disease.

Bone marrow histopathology reveals Gaucher cells (kerasin-laden histiocytes). Significant replacement of liver parenchyma by Gaucher cells leads to hepatomegaly. The course of the disease includes regenerating nodules and hepatic fibrosis, leading to cirrhosis and portal hypertension.18 Splenic manifestations include infarcts and focal clusters of glucosylceramide-laden cells. Bone complications as a result of marrow replacement are common, including pathologic fractures, avascular necrosis, and osteomyelitis.

Treatment: Treatment of Gaucher disease with enzyme replacement therapy is possible.18 In general, the degree of liver and spleen enlargement correlates with disease severity. Thus quantification of hepatosplenomegaly (i.e., measurement of liver and spleen volume) has been used to determine treatment response.20 Liver volume can be measured with sonography, CT, or MRI. The prognosis varies with the type, extent, and severity of disease.

α1-Antitrypsin Deficiency

Imaging: Infants presenting in the first months of life with elevated direct bilirubin levels may undergo scintigraphy with the intention of excluding biliary atresia. In these cases, scintigraphy cannot distinguish biliary atresia from α1-antitrypsin deficiency because both conditions may show good hepatocyte uptake without biliary excretion as a result of a paucity of intralobular bile ducts in some cases of α1-antitrypsin deficiency (e-Fig. 90-7).22 Sonographic correlation may be helpful because infants with α1-antitrypsin deficiency generally have normal sonographic findings of the liver and gallbladder. Abdominal cross-sectional imaging in older children typically reveals nonspecific findings of cirrhosis.23

Wilson Disease

Overview: Wilson disease (hepatolenticular degeneration) is a rare autosomal-recessive disorder of copper metabolism localized to chromosome 13, and in which hepatic excretion of copper into the biliary system does not occur. Normally 95% of copper in the body is bound to the serum protein ceruloplasmin.24 With copper toxicosis, accumulation begins in the liver, and when its copper binding capacity is reached, the basal ganglia, renal tubules, corneas, bones, joints, and parathyroid glands may be affected.

Imaging: Hepatic changes are poorly seen with cross-sectional imaging because multiple processes are occurring in the liver simultaneously: copper accumulation, fatty replacement, hepatitis, cirrhosis, and liver necrosis. The liver is hyperechoic on sonography. Copper has a high atomic number, which increases attenuation on CT. However, hepatic attenuation usually remains normal because of concurrent steatosis, which lowers attenuation and thus may negate the hyperattenuation of copper (e-Fig. 90-8).16 Hepatic MRI early in the course of the disease demonstrates hyperintensity on T1-weighted images and hypointensity on T2-weighted images, but these changes may be overshadowed once cirrhosis supervenes.16,26,27

Cirrhosis

Clinical Presentation: In children, cirrhosis is the result of many different disease processes, including biliary, postnecrotic, and metabolic causes (Box 90-2). Cirrhosis traditionally has been classified into three main categories: micronodular (Laënnec), with equal-sized nodules up to 3 mm; macronodular (postnecrotic), with variable-sized nodules ranging from 3 mm to 3 cm; and mixed cirrhosis.28,29 Micronodular cirrhosis in children is caused by disorders such as biliary obstruction, hemochromatosis, and venous outflow obstruction; macronodular cirrhosis results from disorders such as viral hepatitis, Wilson disease, and α1-antitrypsin deficiency.

Imaging: The sonographic features of cirrhosis include heterogeneous echogenicity of the liver and hepatic surface irregularity (e-Fig. 90-9, A). Other findings include regenerative nodules that may have relatively decreased echogenicity, a smaller right hepatic lobe, and compensatory enlargement of the caudate lobe and left lateral segment. Findings of portal hypertension often are seen, with collateral vessels and hepatofugal flow in the portal vein on Doppler interrogation.28,30

CT shows a small or normal-sized liver with surface nodularity and heterogeneous attenuation that is exaggerated after the administration of contrast material. Decreased attenuation in areas of fatty replacement and normal attenuation in areas of fibrosis and regenerating nodules are common. CT also may demonstrate findings of portal hypertension with development of collateral pathways including coronary to gastroesophageal, paraumbilical, splenorenal, gastrorenal, and hemorrhoidal varices (Fig. 90-9, B).28,30

Typical findings on MRI include morphologic changes already described on ultrasound and CT. Regenerating nodules typically are hypointense to liver on T2-weighted sequences and variable on T1-weighted sequences. No enhancement occurs on postcontrast arterial phase imaging, although enhancement may occur on delayed images when hepatobiliary-specific agents are used (Fig. 90-10). Dysplastic nodules have variable signal intensity on T1-weighted images. Hypointense signal on T2-weighted images is seen in low-grade dysplastic nodules, and hyperintense signal is present in high-grade dysplastic nodules. On postcontrast imaging, low-grade dysplastic nodules are indistinguishable from regenerating nodules, and high-grade dysplastic nodules are indistinguishable from well-differentiated hepatocellular carcinoma (HCC).29 HCC complicating cirrhosis is characterized by variable signal on T1-weighted images and a hyperintense signal on T2-weighted images, in addition to arterial enhancement after administration of contrast material and rapid washout during the portal venous phase.29,30

Treatment: Complications of cirrhosis include ascites, portal hypertension, and HCC. Mortality most often is related to bleeding esophageal varices, hepatorenal syndrome, spontaneous bacterial peritonitis, and treatment related to ascites. Imaging follow-up and surveillance is performed with ultrasound. Further workup with contrast-enhanced MRI is performed when enlarging or suspicious nodules and/or increasing α-fetoprotein levels are present.29,31

References

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