Monocyte maturation

Published on 04/03/2015 by admin

Filed under Hematology, Oncology and Palliative Medicine

Last modified 22/04/2025

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6 Monocyte maturation

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FIGURE 6–1 Monocyte sequence—monoblast.

Monoblast

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FIGURE 6–2A Monoblast.

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FIGURE 6–2B Schematic of monoblast.

Size:

12-18 μm

Nucleus:

Round to oval; may be irregularly shaped

Nucleoli:

1-2; may not be visible

Chromatin:

Fine

Cytoplasm:

Light blue to gray

Granules:

None

N/C RATIO:

4:1

Reference interval:

Bone marrow:

Not defined

Peripheral blood:

None

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FIGURE 6–3 Monocyte sequence—promonocyte.

Promonocyte

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FIGURE 6–4A Promonocyte.

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FIGURE 6–4B Schematic of promonocyte.

Size:

12-20 μm

Nucleus:

Irregularly shaped; folded; may have brainlike convolutions

Nucleoli:

May or may not be visible

Chromatin:

Fine to lacy

Cytoplasm:

Light blue to gray

Granules:

Fine azurophilic (burgundy colored)

N/C RATIO:

2-3:1

Reference interval:

Bone marrow:

<1%

Peripheral blood:

0%

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FIGURE 6–5 Monocyte sequence—monocyte.

Monocyte

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FIGURE 6–6A Monocyte.

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FIGURE 6–6B Schematic of monocyte.

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FIGURE 6–6C Electron micrograph of monocyte (×16,500). Specimens for electron microscopy are prepared by embedding tissue in a suitable medium, such as resin. Ultra-thin cross-sections are then prepared. Because this image shows a cross-section, the lobes of the nucleus appear to be separate, but they are not.

Size:

12-20 μm

Nucleus:

Variable; may be round, horseshoe shaped, or kidney shaped; often has folds producing “brainlike” convolutions

Nucleoli:

Not visible

Chromatin:

Lacy

Cytoplasm:

Blue-gray; may have pseudopods

Granules:

Many fine granules frequently giving the appearance of ground glass

Vacuoles:

Absent to numerous

N/C RATIO:

Variable

Reference interval:

Bone marrow:

2%

Peripheral blood:

3% to 11%

Refer to Table 1-1 for more examples.

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FIGURE 6–7 Monocyte sequence—macrophage.

Macrophage (histiocyte)

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FIGURE 6–8 Macrophage. Bone marrow (A) (×500), (B) (×1000).

Size:

15-80 μm

Nucleus:

Eccentric, kidney or egg-shaped, indented, or elongated

Nucleoli:

1-2

Chromatin:

Fine, dispersed

Cytoplasm:

Abundant with irregular borders; may contain ingested material

Granules:

Many coarse azurophilic (burgundy-colored)

Vacuoles:

May be present

Reference interval:

Macrophages reside in tissues, such as bone marrow, spleen, liver, lungs, and others. Rarely, they are seen in the peripheral blood during severe sepsis.

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FIGURE 6–9 Monocyte sequence with (A) monoblast, (B) promonocyte, (C) monocyte, and (D) macrophage.

All photomicrographs are ×1000 original magnification with Wright-Giemsa staining unless stated otherwise.

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