Lumbar Puncture and Imaging Studies

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Chapter 20 Lumbar Puncture and Imaging Studies

Lumbar Puncture

Neurologists often obtain cerebrospinal fluid (CSF) by performing a lumbar puncture (LP) – one of the oldest neurologic tests still employed – under a variety of clinical circumstances. When patients have at least two elements of the relatively common triad of headache, fever, and nuchal rigidity, neurologists usually perform an LP to look for meningitis, subarachnoid hemorrhage, or other inflammatory conditions affecting the central nervous system (CNS). They also perform an LP in cases of dementia attributable to infectious illnesses. In Creutzfeldt–Jakob disease (CJD), the CSF almost always contains 14-3-3 protein; in subacute sclerosing panencephalitis, antimeasles antibodies; in acquired immunodeficiency syndrome (AIDS), retrovirus markers; and other infectious illnesses, such as cryptococcal or tuberculous meningitis, herpes simplex encephalitis, and neurosyphilis, specific antigens or DNA detectable by polymerase chain reaction. In another particularly useful test, neurologists send CSF for the JC virus in patients with AIDS who have a cerebral lesion. If present, the JC virus indicates that the diagnosis is progressive multifocal leukoencephalopathy (PML) (see Fig. 15-10). Moreover, a positive test obviates the need for a brain biopsy. Neurologists also test the CSF of patients suspected of having multiple sclerosis (MS) for oligoclonal bands and myelin basic protein (see Chapter 15). In Alzheimer disease, the CSF contains increased levels of tau protein but decreased levels of β-amyloid and Aβ42 peptide.

Diagnosing neurologic illnesses sometimes rests on abnormalities of the CSF profile, which comprises the CSF color, red and white blood cell count, and concentrations of protein and glucose (Table 20-1). For example, most infectious or inflammatory CNS illnesses cause a CSF pleocytosis (increase in the CSF white blood cell count). In these illnesses, a rise in protein concentration parallels CSF pleocytosis, and, in their hallmark, glucose concentration falls to abnormally low levels. Bacterial meningitis accentuates that profile: CSF pleocytosis is markedly elevated, with a predominance of polymorphonuclear instead of lymphocytic cells, and the glucose concentration can fall to undetectable levels. Cultures of virus, fungus, and Mycobacterium may require 1–3 weeks to identify an organism, but sometimes antigen testing can immediately indicate bacterial and nonbacterial organisms. As an exception to the general observation that infectious and inflammatory conditions produce CSF pleocytosis, in Guillain–Barré syndrome, CSF contains a markedly elevated protein concentration but little or no increase in the white cell content (the “albumino-cytologic disassociation,” see Chapter 5).

Despite the potential contribution of CSF examination, certain circumstances contraindicate an LP. For example, neurologists do not perform one when patients have a sacral decubitus ulcer because the LP needle might drive bacteria into the spinal canal and infect the CSF. In addition, neurologists insert the LP needle only below the first lumbar vertebra, the lower boundary of the spinal cord, to prevent spinal cord injury.

An intracranial mass lesion is one of the most common contraindications to an LP. This prohibition is based on the fear that an LP could suddenly reduce pressure in the spinal canal, allowing the unopposed force of a cerebral mass to lead to transtentorial herniation (see Fig. 19-3). Moreover, a CSF examination would not help in diagnosing most mass lesions because their CSF profiles are not distinctive. Although increased intracranial pressure without an associated mass lesion defines idiopathic increased intracranial hypertension (pseudotumor cerebri, see Chapter 9), in this setting neurologists perform LPs with impunity for diagnosis and occasionally for treatment. Overall, unless neurologists suspect acute bacterial meningitis or subarachnoid hemorrhage, in which case rapid diagnosis is crucial, they usually do not perform an LP or they postpone it until after imaging studies have excluded an intracranial lesion.

Another potential problem with an LP occurs when trauma during the procedure causes blood to mix with the CSF, which may falsely indicate a subarachnoid hemorrhage or other intracranial source. To distinguish blood induced by the procedure, laboratories centrifuge bloody CSF. Xanthochromia (Greek, xanthos yellow + chroma color) in the supernatant means that intracranial bleeding took place several hours before the LP and that the red blood cells gave rise to the yellow pigment. In contrast, a clear supernatant means that the LP gave rise to the blood in the CSF.

Imaging Studies

Although computed tomography (CT) and magnetic resonance imaging (MRI) should not enslave physicians, they undeniably provide extraordinarily accurate diagnoses. Each technique has given clinical neurology a quantum leap forward. In fact, Drs. Allan M. Cormack and Godfrey N. Hounsfield garnered Nobel prizes in 1979 for the development of CT, and Dr. Paul C. Lauterbur and Sir Peter Mansfield the prizes in 2003 for their discoveries concerning MRI.

Conceding that in many situations these imaging studies surpass the reliability of their neurologic examination, neurologists routinely order CT and MRI to evaluate patients’ dementia, aphasia, neuropsychologic deficits, seizures, and other conditions. Even in cases of apparent delirium, they order it to exclude an underlying structural lesion. Neurologists also use imaging studies to follow the course of certain illnesses, such as brain tumors and MS, because their clinical manifestations often fail to reflect disease activity as reliably as imaging studies.

On the other hand, neurologists do not routinely order imaging studies in evaluating patients with sleep disturbances, absence seizures, cluster and migraine headaches, Parkinson disease, tics, or essential tremor. They also find that imaging studies, while revealing the effects of certain illnesses on the brain and providing academically interesting information, do not help with patients’ diagnosis or management. For example, in autism, dyslexia and other learning disabilities, attention deficit hyperactivity disorder, Tourette disorder, Rett syndromes, and most psychiatric disorders, imaging studies usually show normal brains, small brains, or regional variations, such as cerebellar atrophy. These findings have neither the sensitivity nor specificity to offer diagnostic help.

When neurologists consult on psychiatric patients, they often recommend imaging studies, among other tests, for patients who have a first episode of psychosis, atypical psychosis, major depression after age 50 years, episodic behavioral disturbances, and, in some cases, anorexia. They also suggest imaging studies for patients prior to electroconvulsive treatment for several reasons. A CT or MRI might detect lesions that could explain the psychiatric symptoms without producing overt physical deficits, such as neurocysticercosis or extensive cerebral demyelination. Likewise, cerebral lesions may provoke status epilepticus or transtentorial herniation. Although helpful in many circumstances, imaging studies often fail to clarify the relationships, if any, between psychiatric symptoms and many common abnormalities that the studies uncover, such as cerebral atrophy, mild communicating hydrocephalus, small cerebral lesions, subcortical hyperintensities, and congenital abnormalities.

Computed Tomography

Using beams of ionizing radiation, which are essentially X-rays, CT generates images of the brain, other soft tissues, and skull. CT displays structures increasingly more radiodense than brain, such as tumors, blood, bone, calcifications, and surgical devices, in gradations increasingly closer to white than black. Similarly, it shows structures increasingly less radiodense than the brain, particularly the CSF-filled ventricles, in gradations increasingly closer to black. Thus, it shows in dark to black gradations several common lesions characterized by the absence of acute blood, such as cerebral infarctions, chronic subdural hematomas, edema surrounding tumors, and the center of cystic lesions. Likewise, it shows in light to white gradations several common lesions characterized by calcium or excessive blood, such as calcifications in the choroid plexus or meningiomas, acute subarachnoid, subdural, or intracerebral hemorrhages, and intraventricular shunts. By manipulating the software, CT and MRI can display the brain from three major perspectives: transaxial (axial, the conventional top-down view), coronal (front-to-back view), and sagittal (side view).

Although lacking fine detail, CT can clearly reveal changes in major structures (Fig. 20-1). It shows generalized cerebral atrophy, such as occurs in advanced age or Alzheimer disease (Figs 20-2 and 20-3), and atrophy of a particular region, such as with porencephaly (Fig. 20-4), Huntington disease (Fig. 20-5), and frontotemporal dementia (Fig. 20-6). Similarly, it shows expansion of the ventricles – hydrocephalus – not only as a consequence of generalized atrophy (hydrocephalus ex vacuo, Fig. 20-3), but also from normal-pressure hydrocephalus (Fig. 20-7) and CSF obstructions (obstructive hydrocephalus) (see later). CT readily detects large lesions, such as primary and metastatic tumors (Fig. 20-8). CT will also reveal subdural hematomas, except perhaps for isodense ones (Fig. 20-9). CT is even superior at finding dense, calcium-laden meningiomas (Fig. 20-10). It can show numerous small lesions, such as in toxoplasmosis (Fig. 20-11) and cysticercosis (Fig. 20-12), with the detail necessary for a firm diagnosis. (Cysticercosis, which is caused by the parasite Taenia solium, is the most common cerebral mass lesion in South and Central America.)

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FIGURE 20-2 This computed tomography (CT) scan illustrates generalized cerebral atrophy. (See Fig. 20-18 for magnetic resonance imaging appearance of cerebral atrophy.) Because of atrophy, the gyri shrink, sulci expand, cerebral cortex retracts from the inner table of the skull and from the falx (arrow), and ventricles expand (see later). Cerebral atrophy, as pictured in this case, represents a normal concomitant of old age. Although cerebral atrophy is associated with Alzheimer disease, vascular cognitive impairment, trisomy 21, alcoholism, neurodegenerative illnesses, and treatment-resistant schizophrenia, it is not invariably associated with dementia.

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FIGURE 20-5 A, This computed tomography (CT) scan shows the characteristic abnormality of Huntington disease: the anterior horns of the lateral ventricles are convex (bowed outward) because of atrophy of the caudate nuclei (arrows). Contrast that convex shape of the ventricles in Huntington disease to the concave shape seen in normal individuals (see Figs 20-1B and 20-17) and in those with cerebral atrophy and hydrocephalus ex vacuo (see Figs 20-2, 20-3, and 20-18). In addition to the caudate atrophy, Huntington disease, like many other neurodegenerative illnesses, causes cortical atrophy with widened sulci and enlarged ventricles. B, This coronal view of the MRI of the same patient also shows the convex expansion of the lateral ventricles, large sulci, and widened sylvian fissures (S).

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FIGURE 20-8 A, Computed tomography (CT) scan shows a glioblastoma with its characteristic white, contrast-enhanced ring (right) and black border of edema (arrows). (See Fig. 20-20 for magnetic resonance imaging showing a glioblastoma.) B, With contrast enhancement, CT shows several metastatic cerebral tumors (arrows).

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FIGURE 20-12 This computed tomography (CT) scan shows multiple cerebral cysticercosis lesions in views through the cerebrum (A) and cerebral cortex (B) and an enlargement of the frontal cortex lesion (C). These lesions, in contrast to toxoplasmosis (see Fig. 20-11), are usually situated in the cerebral cortex, contain calcification, and lack surrounding edema. Even though each cyst is small, together they exert a substantial cumulative mass effect and irritate the surrounding cerebral cortex. Their tendency to irritate the cerebral cortex explains why patients with cysticercosis often first come to medical attention because of seizures.

CT can also show strokes that are large established infarctions and those that are hemorrhagic (Fig. 20-13); however, MRI can better locate ones that are small or acute. CT is invaluable in the special situation of cerebellar hemorrhage, where rapid diagnosis is essential to prevent brainstem compression and obstructive hydrocephalus (Fig. 20-14).

Physicians routinely obtain head CT in suspected child abuse because 80% of cases include nonaccidental head injury (NAHI). Moreover, up to 50% of cases of NAHI result in neurologic deficits and 30% in fatality. Subdural hematomas – bilateral and of different ages – are the hallmark of NAHI.

The administration of an intravenous contrast solution during CT increases the density of blood-filled structures and whitens their image. This technique, contrast enhancement, highlights vascular structures, such as arteriovenous malformations (AVMs), glioblastomas, and membranes surrounding chronic subdural hematomas and cystic lesions (Fig. 20-15).

Although MRI holds many advantages, CT remains clinically indispensable, less expensive than MRI, and highly reliable. It is particularly valuable during emergencies, when speed is critical and gross anatomical pictures suffice. For example, the procedure, which may take as little as 8 minutes, satisfactorily reveals lesions that require immediate attention, such as epidural and acute subdural hematomas, large cerebellar hemorrhages, obstructive hydrocephalus, and subarachnoid hemorrhage. Also, patients with pacemakers, defibrillators, and other indwelling metallic devices, and those with claustrophobia can undergo CT but not MRI.

On the other hand, undergoing a CT exposes any patient to ionizing radiation. This radiation exposure potentially endangers children and adolescents because their dosage is relatively greater than for adults and their skull is thinner. Giving roughly the radiation of a skull X-ray series, a single head CT adds a measurable risk (0.07%) to the child’s lifetime cancer risk. Furthermore, dental X-rays and, more so, dental CTs add to their lifetime radiation exposure. Another consideration in children is that, because ocular lenses are highly susceptible to radiation, CT adds to the risk of developing radiation-induced cataracts.

Magnetic Resonance Imaging

In MRI, a powerful magnet forces protons to spin with their axes parallel to the magnetic field. Then radiofrequency (RF) pulses align the axes. After each RF pulse, the protons resume their original alignment (“relax”) within the magnetic field and thereby emit energy. Different tissues emit characteristic, identifiable energy signals.

In the brain, hydrogen nuclei (protons) in water-containing tissues emit most of the signal. The differences in water content of tissues in various areas of the brain result in signals of different intensity. Sophisticated software converts them into images (the scans).

MRI offers several advantages over CT in addition to not exposing the patient to ionizing radiation. Because the resolution of MRI surpasses that of CT, its images provide finer detail (Fig. 20-16), more vivid displays of neuroanatomy (Fig. 20-17), and better illustrations of large common structural changes, such as atrophy (Fig. 20-18) and hydrocephalus (see Figs 20-5, 20-7, and 20-19). It also shows fine detail of mass lesions, such as glioblastomas and strokes (Figs 20-20 through 20-24). With diffusion-weighted images, a standard sequence of a routine study, MRI can show cerebral infarctions not only with fine detail, but, unlike CT, also almost immediately after their onset (see Fig. 20-24). MRI, but not CT, can support the diagnosis of illnesses that alter cerebral or spinal cord white matter, i.e., leukoencephalopathies, such as many congenital storage diseases, MS (Fig. 20-25), PML, and hydrocarbon solvent abuse. In MS, neurologists rely on MRI to confirm the diagnosis, establish the extent, and subsequently detect subclinical as well as clinical progression. It also shows characteristic changes, although not diagnostic ones, in prion illnesses, such as CJD and fatal familial insomnia (see Chapter 17).

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FIGURE 20-17 A, A magnetic resonance imaging (MRI) sagittal view of a normal brain reveals exquisitely detailed cerebral gyri and sulci, the corpus callosum (CC), and three major structures of the posterior fossa, the pons (P), medulla (M), and cerebellum (C). The anterior portion of the corpus callosum is the genu, and its posterior portion, the splenium. In addition, it shows the cervical–medullary junction and various nonneurologic soft-tissue structures. Note that the medulla remains above the foramen magnum. B, The coronal view reveals the corpus callosum, the “great commissure,” which spans and interconnects the cerebral hemispheres. The white matter of the corpus callosum and subcortical cerebral hemispheres is distinct from the ribbon of overlying gray matter. The anterior horns of the lateral ventricles, with their concave lateral borders, are beneath the corpus callosum and medial to the caudate nuclei (see Fig. 18-1, A). The cerebral cortex around the left (dominant) sylvian fissure (arrow), including the planum temporale, is usually more convoluted than that around the right (nondominant). The convolutions confer greater cortical area for language function on the dominant hemisphere. The frontal lobe is above the sylvian fissure, and the temporal lobe is below. The medial inferior surface of the temporal lobe (T), which is the origin of most partial complex seizures, is sequestered by the bulk of the temporal lobe above and below the sphenoid wing anteriorly. It is far from the sites of conventional scalp electroencephalogram (EEG) electrodes.

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FIGURE 20-18 Four magnetic resonance imaging (MRI) images of cerebral atrophy can be contrasted to the normal brain (see Fig. 20-17). MRI emphasizes cerebral atrophy because it does not detect the cortical bone of the skull, which emits almost no signal because it contains virtually no water. However, the scalp emits a signal because it contains blood, fat, and other water-containing soft tissues. A, In this axial view through the cerebral hemispheres, the cerebrospinal fluid (CSF), which is dark, fills the dilated lateral ventricles and sulci. B, In a view that shows the surface of the brain, computed tomography demonstrates the thin gyri. To fill the void left by the atrophied gyri, copious amounts of CSF fill the sulci and cover the cortex. C, In a sagittal view, the MRI shows thin, ribbon-like frontal lobe gyri (large arrows) and the less atrophied parietal lobe gyri (small arrow). The corpus callosum, pons, and cerebellum stand out. The tentorium, appearing as a straight line, is situated above the cerebellum. D, This coronal view through the frontal lobes shows typical manifestations of cerebral atrophy: (1) dilated lateral ventricles; (2) an enlarged third ventricle; (3) enlargement of the anterior interhemispheric fissure because of separation of the medial surfaces of the frontal lobes; and (4) dilated sylvian fissures with the atrophic temporal lobe (t) below and the atrophic frontal lobe above.

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FIGURE 20-19 This magnetic resonance imaging study shows a coronal view of the brain of a patient with normal-pressure hydrocephalus. (See Fig. 20-7 for a comparable computed tomography scan.) It demonstrates the classic findings: dilation of the lateral ventricles, their temporal horns (white arrows), and the third ventricle (double arrows), and absence of cerebral atrophy.

MRI holds another advantage because most of the skull is composed of cortical bone, which contains no water: the skull does not produce linear streak artifacts that obscure images – a common problem in CT. The lack of artifact allows MRI to generate detailed images of structures in bony casings, such as the acoustic nerves, cerebellum and other posterior fossa contents, the pituitary gland, and the spinal cord. Neurologists require MRI in diagnosing mesial temporal sclerosis (Fig. 20-26) and planning epilepsy surgery. It is also indispensable in identifying acoustic neuromas (Fig. 20-27). However, because it does not detect lesions with little or no water content, MRI may fail to display skull fractures.

Administration of “paramagnetic” contrast solutions, such as gadopentetate (gadolinium), can enhance intracranial abnormalities. Although they do not cross the intact blood–brain barrier, contrast solutions highlight lesions that disrupt the barrier, such as neoplasms, abscesses, active MS plaques, and acute infarctions.

Despite its greater resolution, MRI is no more effective than CT in diagnosing several important illnesses, including Alzheimer disease and childhood neuropsychiatric conditions. Moreover, it has some disadvantages. One problem is that for 30–40 minutes, patients remain entirely within the bore of the MRI magnet – an intimidating long, narrow tunnel, with a diameter only slightly wider than their body. Even excluding patients with known claustrophobia, at least 10% of the remainder, sometimes in utter panic, abort the procedure. Taking a benzodiazepine and wearing a sleep mask alleviates enough anxiety to allow many mildly claustrophobic patients to remain in the machine.

A potentially life-threatening problem with MRI is that the magnet forcefully attracts ferrous metals. Metallic objects inadvertently brought into the room have formed deadly missiles. Pacemakers, implanted hearing devices, intracranial aneurysm clips manufactured before 1993, and other medical devices might be dislodged or destroyed if the patient were exposed to the intense magnetic field.

As an expectable corollary of the large number of studies and the high resolution of MRI, many studies detect abnormalities that have no established clinical significance. For example, MRI in 4% of asymptomatic individuals aged 45–60 years old and in almost 20% of those 75–97 years old shows small hyperintense lesions, which neurologists dub “unidentified bright objects (UBOs).” Neurologists variously attribute these UBOs to migraine, small strokes, small MS plaques, dilated CSF spaces, or normal age-related changes. MRI also detects innocuous small cerebral artery aneurysms and meningiomas that bear no relationship to patients’ symptoms.

Other Applications of Magnetic Resonance

With the appropriate software, magnetic resonance can generate images of intracranial and extracranial cerebral vessels. This technique, magnetic resonance angiography (MRA), can display highly accurate images of the carotid and vertebral arteries (see Figs 11-2 and 20-28). It can detect aneurysms, AVMs, and other vascular malformations. Because it can outline internal carotid artery stenosis, plaques, and dissections, MRA eliminates the need for conventional carotid angiography, which can be hazardous and painful.

The remarkable sensitivity of MRI has led to functional MRI (fMRI), a technique that displays gross metabolic activity. Based on different properties of blood in its oxygenated and deoxygenated states, fMRI exploits small increases in blood flow and oxygen metabolism during cerebral activity. It highlights regions of the brain receiving sensory stimuli, initiating physical activity, imagining sensory or physical experiences, and performing cognitive processes – activities that increase metabolic demands. For example, it can detect language circuits. Thus, fMRI may eventually replace the Wada test in epilepsy surgery candidates (see Chapter 8).

Magnetic resonance spectroscopy (MRS) detects the chemical composition of cerebral tissues and lesions. The technique’s software suppresses water-generated signals and then analyzes the remaining ones to determine the presence and concentration of choline, creatine, N-acetylaspartate, lactic acid, lipids, and other chemicals. Preliminary work has shown that MRS can characterize tumors, abscesses, other lesions, mitochondrial encephalopathies, and degenerative diseases by the presence and relative concentrations of these substrates.

Another application of magnetic resonance, diffusion tensor imaging (DTI), shows normal and abnormal flow of water molecules. DTI measures anisotropy, which consists of the asymmetrical diffusion of water molecule motion. Among its many uses, DTI dramatically and in multiple colors shows white-matter tracts. It readily detects traumatic brain injury and toxic insults even in cases without overt abnormality on conventional MRI.

Positron Emission Tomography

In contrast to CT and MRI, which can provide exquisitely detailed images of CNS anatomy, positron emission tomography (PET) provides a rough, relatively low-resolution picture of metabolic activity, chemistry, and physiology of the brain. In other words, PET illustrates brain function rather than structure. PET relies on positron-emitting, biologically active radioisotopes (radioligands) produced in cyclotrons and incorporated into organic molecules. The radioligands, which are inhaled or injected intravenously, undergo metabolism in the brain and emit positrons. The reaction between positrons and electrons produces photons, which PET detects and transforms into images.

Most PET studies measure the metabolism of a substitute for glucose, fluorine-18-labeled fluorodeoxyglucose (FDG). Like glucose, FDG is absorbed into the brain and metabolized. The metabolism of FDG emits positrons at a rate that parallels cerebral glucose metabolism. Similarly, metabolism of oxygen-15-labeled water reflects cerebral blood flow and metabolism of fluorine-18-labeled fluorodopa reflects dopamine metabolism. PET using radioligands for serotonin, gamma-aminobutyric acid, and acetylcholine permits visualization of the distribution and activity of their receptors. All these radioligands have a brief half-life. For example, oxygen-15 has a half-life of 2 minutes, and fluorine-18 less than 2 hours.

Neuroscientists use PET to analyze cerebral metabolism during normal activities, administration of medications, and several illnesses. It offers valuable imaging in some varieties of epilepsy, neurodegenerative illnesses, and structural lesions. In epilepsy, PET images show that, in complex partial epilepsy, the affected temporal lobe is generally hypoactive during the interictal period but hyperactive during seizures. Determining whether the temporal lobe is epileptogenic by this method, which is complementary to electroencephalography and fMRI, helps decide if a temporal lobectomy would benefit a patient with intractable epilepsy (see Chapter 10).

PET is also helpful in studying several neurodegenerative illnesses. In Alzheimer disease, PET shows decreased cerebral metabolism, especially in the parietal and frontal lobes’ association areas (Fig. 20-29). It can also help distinguish vascular neurocognitive disorder and frontotemporal dementia from Alzheimer disease. More importantly, using a special amyloid-binding ligand Pittsburgh Compound B, PET can localize and quantitate amyloid deposition, which allows for a diagnosis of Alzheimer disease in its presymptomatic as well as symptomatic stages (see Chapter 7). Similarly, PET abnormalities may appear before either clinical signs or MRI abnormalities in Parkinson and Huntington diseases. It can monitor the progression of these illnesses.

Although useful when the diagnosis rests on regional changes in metabolic activity, PET cannot reliably detect or classify brain tumors because the brain is so highly metabolic that any increased activity generated by a tumor would be lost in the background levels. Moreover, its resolution does not allow for inspection of a lesion’s anatomy. However, PET can make valuable contributions in certain situations because it can distinguish between tumors and some nonmalignant conditions. For example, PET can distinguish between recurrent brain tumors and radiation necrosis. Also, as a complement to CSF testing for the JC virus in the diagnosis of PML, PET can also distinguish PML from lymphoma because PML lesions are hypoactive and lymphomas are hyperactive.