Eosinophil maturation

Published on 04/03/2015 by admin

Filed under Hematology, Oncology and Palliative Medicine

Last modified 22/04/2025

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7 Eosinophil maturation

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FIGURE 7–1 Eosinophilic sequence—eosinophilic myelocyte.

Eosinophilic myelocyte

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FIGURE 7–2A Eosinophilic myelocyte.

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FIGURE 7–2B Electron micrograph of eosinophilic granules to demonstrate crystalline structure of granules.

Size:

12-18 μm

Nucleus:

Round to oval; may have one flattened side

Nucleoli:

Usually not visible

Chromatin:

Coarse and more condensed than promyelocyte

Cytoplasm:

Colorless to pink

Granules:

Primary:

Few to moderate

Secondary:

Variable number; pale orange to dark orange; round; appear refractile

N/C RATIO:

2:1 to 1:1

Reference interval:

Bone marrow:

0% to 2%

Peripheral blood:

0%

Note:

This chapter begins with the image of the myelocyte, rather than the blast, because it is at the myelocyte stage that secondary granules, which define a cell as an eosinophil, first appear.

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FIGURE 7–3 Eosinophilic sequence—eosinophilic metamyelocyte.

Eosinophilic metamyelocyte

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FIGURE 7–4 Eosinophilic metamyelocyte.

Size:

10-15 μm

Nucleus:

Indented; kidney bean shape; indentation is less than 50% of the width of the hypothetical round nucleus

Nucleoli:

Not visible

Chromatin:

Coarse, clumped

Cytoplasm:

Colorless

Granules:

Primary:

Few

Secondary:

Many pale orange to dark orange; appear refractile

N/C RATIO:

1.5:1

Reference interval:

Bone marrow:

0% to 2%

Peripheral blood:

0%

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FIGURE 7–5 Eosinophilic sequence—eosinophilic band.

Eosinophilic band

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FIGURE 7–6 Eosinophilic band.

Size:

10-15 μm

Nucleus:

Constricted but no threadlike filament: indentation is more than 50% of the width of a hypothetical round nucleus

Note:

Chromatin must be visible in constriction

Nucleoli:

Not visible

Chromatin:

Coarse, clumped

Cytoplasm:

Colorless, cream-colored

Granules:

Primary:

Few

Secondary:

Abundant pale to dark orange; appear refractile

N/C RATIO:

Cytoplasm predominates

Reference interval:

Bone marrow:

0% to 2%

Peripheral blood:

Rarely seen

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FIGURE 7–7 Eosinophilic sequence—eosinophil.

Eosinophil

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FIGURE 7–8A Eosinophil.

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FIGURE 7–8B Fractured eosinophil.

Size:

12-17 μm

Nucleus:

2-5 lobes connected by thin filaments without visible chromatin; majority of mature cells have 2-3 lobes

Nucleoli:

Not visible

Chromatin:

Coarse, clumped

Cytoplasm:

Cream-colored; may have irregular borders

Granules:

Primary:

Rare

Secondary:

Abundant pale orange to dark orange; round

N/C RATIO:

Cytoplasm predominates

Reference interval:

Bone marrow:

0% to 3%

Peripheral blood:

0% to 5%

Refer to Table 1-1 for more examples.

Note:

Eosinophils are fragile and may easily fracture when preparing blood film.

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FIGURE 7–9 Eosinophilic sequence with (A) eosinophilic myelocyte, (B) eosinophilic metamyelocyte, (C) eosinophilic band, and (D) eosinophil.

All photomicrographs are ×1000 original magnification with Wright-Giemsa staining unless stated otherwise.

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