Benign liver lesions

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Benign liver lesions

Introduction

Benign liver lesions are common and may be difficult to differentiate from primary and secondary hepatic tumours. Such may be identified as an incidental finding when radiological investigation is undertaken for unrelated intra-abdominal disease or when coexistent hepatic pathology is present, giving rise to problems of diagnosis and management. Although these lesions may be of congenital origin, most are of unknown aetiology. They generally are asymptomatic but, since they are often slow growing, they may produce symptoms caused by mass effect or compression of adjacent organs. Rarely, such lesions may give rise to acute symptoms resulting from necrosis, thrombosis, haemorrhage or rupture.

Routine liver function tests are invariably normal unless the lesion compresses the biliary confluence or common bile duct, and are therefore of value in guiding the clinician towards a diagnosis of benign disease. Nonetheless, complications such as haemorrhage and necrosis may be associated with increases in serum transaminase levels. Elevation in tumour markers and the development of paraneoplastic syndromes such as erythrocytosis, hyperglycaemia and hypercalcaemia are observed rarely.

Characterisation of hepatic lesions is provided by imaging tests of the liver. Ultrasonography (US), computed tomography (CT) and magnetic resonance imaging (MRI) are the cornerstones of diagnosis and often complement one another. More recently, positron emission tomography (PET) has shown promise. Abdominal US will differentiate cystic forms from solid lesions, whereas CT and MRI using intravenous contrast and delayed imaging will not only detect the number and size of the lesions, but also allow further characterisation. Haemangioma and focal nodular hyperplasia are relatively easily confirmed by modern imaging techniques, whereas differentiation of liver cell adenoma from well-differentiated hepatocellular carcinoma remains challenging.

Biopsy should only be performed in those patients who are being considered candidates for surgical intervention and only where the results of biopsy might influence further management. Biopsy is contraindicated for patients with suspected haemangioma, haemangioendothelioma and cysts suspected of being echinococcal in origin. Needle biopsy or fine-needle aspiration cytology of suspected hypervascular solid tumours may result in haemorrhage, sampling error, misdiagnosis and needle-tract tumour seeding. Tissue from a haemangioma may resemble fibrosis, and focal nodular hyperplasia may resemble cirrhosis. Needle samples of hepatic adenoma may be interpreted as normal tissue and may be difficult to differentiate from well-differentiated hepatocellular carcinoma. In selected patients with tumours of unclear nature, biopsy may be useful by a percutaneous approach for deep-sited lesions or by a laparoscopic approach for superficial and accessible lesions.

The general surgeon should be thoroughly familiar with the gross appearance, clinical significance and natural history of these benign lesions as the treatment strategy may vary from simple observation (of focal nodular hyperplasia) to complex radical hepatic resection (of hepatocellular adenoma). Most symptomatic benign liver lesions are excised; however, hepatic resection, if performed without the proper indications, can prove hazardous.

Classification

Although a variety of benign liver tumours have been described, many are rare and a detailed description of these various lesions is beyond the scope of the current text (Box 4.1). The majority of benign hepatic lesions encountered in clinical practice include haemangioma, liver cell adenoma, focal nodular hyperplasia, bile duct hamartoma and hepatic cysts. For completeness, a brief résumé is provided of less common and miscellaneous lesions that may give rise to diagnostic and management dilemmas.

Haemangiomas

Haemangiomas are the most common benign hepatic tumours of mesenchymal origin. Small capillary haemangiomas are more common than the larger cavernous haemangiomas and are often multiple. Small lesions are asymptomatic and an incidental finding; however, they may give rise to diagnostic difficulty in patients undergoing investigation. Once accurate diagnosis has been made no further therapy is needed. Haemangiomas are probably of congenital origin and do not undergo malignant transformation. The incidence of cavernous haemangioma in autopsy series varies considerably but has been reported to be as high as 8%. These lesions are the second most common hepatic tumour in the USA, exceeded only by hepatic metastases.1 With the more widespread use of sensitive imaging studies of the upper abdomen, the identification of such lesions as an incidental finding will undoubtedly be more common. Cavernous haemangiomas may reach an enormous size, and lesions weighing up to 6 kg are well documented. There is poor agreement in the literature as to the exact definition of what constitutes a giant haemangioma. Some are defined as greater than 4 cm in diameter, others greater than 6 cm. Such haemangiomas are usually solitary, but multiple lesions have been described in about 10% of cases.1 They may be associated with similar lesions in the skin and other organs. Lesions are usually evenly distributed throughout the liver and its substance but large lesions situated peripherally may form a pedicle.

Pathology

Cavernous haemangiomas are seen most frequently in patients in the third to fifth decades of life. They are more common and more likely to become clinically manifest at a younger age in women, are more common with increasing parity and may enlarge during pregnancy.24 This indicates a possible role of female sex hormones in their development, although an association with the oral contraceptive pill has not been proven. The aetiology of liver haemangiomas is still unclear but they may represent benign congenital hamartomas. These lesions appear to grow by progressive ectasia rather than hyperplasia or hypertrophy. At operation, they appear as well-circumscribed, reddish-purple, hypervascular lesions, which may be multilobulated or have a smooth surface. When sectioned, the lesion will partially collapse due to the escape of blood, and it has a honeycombed cut surface. There may be gross evidence of thrombosis, fibrosis or calcification (Fig. 4.1). Microscopically, haemangiomas are composed of cystically dilated vascular spaces, lined by endothelial cells and separated by fibrous septa of varying thickness. There is usually a clear plane between haemangioma and normal liver tissue as these lesions are usually encapsulated by a rim of fibrous tissue.

Clinical presentation

Most haemangiomas are asymptomatic but subcapsular lesions and larger lesions causing compression of adjacent organs may produce clinical features. Symptoms may include vague abdominal pain or fullness, early satiety, nausea, vomiting or fever. Rare presentations include obstructive jaundice, gastric outlet obstruction and spontaneous rupture. Although abdominal pain or discomfort is the most frequent indication for removing a liver haemangioma, it must be remembered that associated pathology may coexist and be the cause of the symptoms. Farges et al.5 reported that 42% of the patients in their series had other pathology, such as gallbladder disease, liver cysts, gastroduodenal ulcers or hiatus hernia. The difficulty of attributing symptoms to the haemangioma is evidenced by the occasional persistence of symptoms after resection.6

Pain related to an uncomplicated haemangioma is likely due to stretching or inflammation of Glisson’s capsule. Occasionally, large lesions located in the left lobe of the liver may cause pressure effects on adjacent structures and infarction or necrosis may account for the sudden onset of pain. Intra-abdominal haemorrhage due to spontaneous or traumatic rupture of haemangioma is a very rare complication.4 A past review of the literature included only 28 reports of spontaneous, life-threatening haemorrhage due to liver haemangiomas, a minimal figure considering the prevalence of the tumour.7 Thrombocytopenia and hypofibrinogenaemia have also been associated with cavernous haemangiomas of the liver (Kasabach–Merritt syndrome), and this effect may be related to consumption of coagulation factors.1

A large haemangioma on the liver edge may be palpable on inspiration. It is difficult to differentiate the consistency of a haemangioma from normal liver through the abdominal wall unless it has calcified or undergone thrombosis or fibrosis. Occasionally, a bruit may be heard over a haemangioma, but this is a non-specific finding. Liver function tests are normal in the patient presenting without complication.

Such lesions are generally hyperechoic on ultrasound examination (Fig. 4.2). Farges et al.5 found the diagnosis to be established by US alone in 80% of patients with haemangiomas smaller than 6 cm. However, this investigation alone cannot differentiate a haemangioma from hepatocellular carcinoma, liver cell adenoma, focal nodular hyperplasia or a solitary metastasis. CT has proven useful in the diagnosis of haemangiomas.8 Prior to intravenous contrast infusion, CT shows the haemangioma to consist of a well-demarcated hypodense mass. After the intravenous injection of contrast medium, serial scans will reveal a zone of progressive enhancement peripherally that varies in thickness and often demonstrates an irregular margin (Fig. 4.3). The centre of the haemangioma remains hypodense and the overall lesion size does not change. Over the past decade MRI has emerged as a highly accurate technique for diagnosing and characterising liver haemangioma, with a reported 90% sensitivity, 95% specificity and 93% accuracy9,10 (Fig. 4.4). Haemangiomas are typically very bright (light bulb sign) on T2-weighted images and show peripheral nodular enhancement on dynamic gadolinium-enhanced T1-weighted images.11 Single-photon emission CT (SPECT) using technetium-99m-labelled red blood cells has been shown to increase the spatial resolution of planar scintigraphy and has been shown to have a sensitivity and accuracy close to that of MRI.12 Fluorodeoxyglucose (FDG)-PET has been reported as useful to differentiate giant hepatic cavernous haemangiomas from malignant hepatic tumours.13 In practice, a combination of these diagnostic modalities is preferred. Superficial lesions may be identified incidentally during abdominal laparoscopic procedures and should be recognised by their gross appearance and characteristic compressibility when gently palpated with a laparoscopic instrument. Needle biopsy of vascular liver lesions should not be performed. Diagnostic uncertainty is seldom a problem with cavernous haemangiomas, except in lesions not large enough to show cavernous characteristics.

Management

A wide range of management strategies from observation to resection has been advocated for such lesions. Simple reassurance should be given to patients in whom small lesions (i.e. < 6 cm) have been detected as an incidental finding. For larger cavernous haemangiomas, consideration should be given to weighing the risk of operation against the natural history of untreated lesions. Trastek et al.4 followed up 34 untreated patients over a maximum period of 15 years. No patient had a lesion that bled, none reported abdominal symptoms and no patient had compromise of quality of life. A further report from the same group, when the observation period had been extended to 21 years, reported two patients with large symptomatic lesions of questionable resectability at initial presentation who remained symptomatic but with little documented growth of the haemangioma. The remainder were asymptomatic and there was no instance of rupture.14 Two more recent longitudinal studies have supported the accepted view that asymptomatic giant haemangiomas of the liver can be managed safely by observation.15,16

Nichols et al.14 reported no operative deaths and the single postoperative complication was a wound infection in 41 patients undergoing resection of such lesions. In a similar series of 69 patients, Weimann et al.17 reported no postoperative deaths and a morbidity rate of 19%. Also in this series were 104 patients with haemangioma and 53 patients with focal nodular hyperplasia who were observed for a median of 32 months (range 7–132 months). There was no evidence of malignant transformation or tumour rupture. Therefore, safe resection is possible but there is no evidence that asymptomatic patients should undergo resection since the risk of rupture is minimal.5,18

When treatment is indicated, because of highly symptomatic or complicated lesions, surgical excision provides the only effective therapy. Reports of the effectiveness of hepatic arterial ligation are anecdotal. Arterial ligation or embolisation may, however, be considered for the temporary control of haemorrhage in exceptional circumstances in order to allow time for transfer of a patient for definitive management in a specialist centre. The benefits of radiation therapy and corticosteroids have not been well documented and are inconsistent. It is possible that the success of non-resectional therapy may well be largely due to the naturally occurring spontaneous involution of these lesions.

The choice of excision requires consideration of the size and anatomical location of the lesion. Haemangiomas can often be enucleated19 to avoid loss of functional liver parenchyma, diminish blood loss and minimise postoperative bile leakage, although in some cases it may be wiser and safer to perform a formal anatomical liver resection. At enucleation, a plane between the lesion and the liver is easily found and this can be developed by blunt dissection. This can be facilitated by the use of the Cavitron ultrasonic surgical aspiration system (CUSA™) with concomitant control of the inflow vessels. Laparoscopic resection of liver haemangioma is increasingly reported,20 and orthotopic liver transplantation has been used successfully to treat symptomatic patients with technically unresectable complicated giant haemangioma.21

Liver cell adenoma

Although liver cell adenoma requires differentiation from any solid hepatic lesion, it is often considered alongside focal nodular hyperplasia.22

Hepatic adenomas arise in otherwise normal liver and present as a focal abnormality or mass. The true prevalence of the disease is difficult to assess but 90% develop in women in the third to fifth decades of life.23 These tumours were rarely reported before 1960, but their apparent increase in incidence since then corresponded with the introduction of oral contraceptives at that time. The causal relationship between liver cell adenoma and oral contraceptives was first suggested by Baum et al.24 in 1973. Ninety per cent of patients with liver cell adenomas have used oral contraceptives and the annual incidence among oral contraceptive users has been reported to be 3–4 per 100 000 if the contraceptives are taken for more than 2 years. The risk of developing a liver cell adenoma increases with the dose and duration of use of the contraceptive preparation.23 Furthermore, pregnancy has been associated with increased symptoms and an increased risk of complications in patients with liver cell adenomas.23,25 The introduction of low-oestrogen-containing contraceptive preparations may result in a reduction in incidence, although adenomas are also associated with non-contraceptive oestrogen use, androgenic steroid use, diabetes, glycogen storage disease, galactosaemia and iron overload. This association implicates altered carbohydrate metabolism in the formation of liver cell adenomas.26

Pathology

Liver cell adenomas are usually solitary, round and occasionally encapsulated. Lesions are soft and smooth surfaced, but occasionally may be pedunculated. The cut surface has a pale yellow fleshy appearance unless haemorrhage and necrosis produce discoloration (Fig. 4.5). They are sharply demarcated from normal liver but without a fibrous capsule. Approximately 12–30% of these tumours are multiple, and if more than 10 adenomas are present, the condition is regarded as liver adenomatosis.27 This may be a separate pathological entity from isolated liver cell adenoma as both sexes are equally affected and oral contraceptive usage is unusual. Microscopically, there are uniform masses of benign-appearing hepatocytes without ducts or portal triads. The hepatocytes appear paler than normal because of increased glycogen or fat content. Venous lakes (peliosis hepatis) are often seen.

Historically, liver cell adenomas have been considered precancerous. Rooks et al.23 reported the finding of hepatocellular carcinoma 5 years after resection of a liver cell adenoma, and other authors have recognised unequivocal areas of hepatocellular carcinoma adjacent to or within liver cell adenomas.23,28,29 Also reported is the development of hepatocellular carcinoma several years after diagnosis of biopsy-proven benign liver cell adenoma.26,30,31 More recent studies have shown that there are different subtypes of liver cell adenoma and that the risk of malignant transformation varies. Liver cell adenoma occurring in men and large tumours are at highest risk, but telangiectatic or unclassified liver cell adenomas have an increased risk whereas steatotic liver cell adenomas have a lower risk.32 Recent innovations in molecular biology and immunohistochemistry have identified β-catenine mutation as a significant risk factor for malignant transformation.33,34

Clinical presentation

These lesions present frequently with abdominal pain from haemorrhage into the tumour or adjacent liver. Some patients develop severe acute abdominal pain due to intraperitoneal rupture and haemoperitoneum, which may present as hypovolaemic shock. The risk of bleeding is reported as 21–50% and is not related to tumour size.32 Up to one-third of patients sense the presence of an abdominal mass. The remainder of adenomas are discovered incidentally at autopsy, laparotomy or during radiological assessment for another problem.

Although the clinical presentation may be suggestive of liver cell adenoma, definitive preoperative diagnosis may be difficult. Liver function tests are generally normal unless tumour necrosis or haemorrhage is present. Anaemia may therefore occur. US can detect small adenomas, which characteristically display a lesion of mixed echogeneity and heterogeneous texture. CT may show evidence of recent haemorrhage or necrosis. Lesions are generally hypodense prior to infusion of contrast medium and demonstrate a wide range of densities after intravenous contrast administration. They often appear as well-demarcated, fat-containing or haemorrhagic lesions on MRI. Conventional radiological imaging may not be able to differentiate between liver cell adenoma and hepatocellular carcinoma; however, promising results have been reported with the use of FDG-PET to differentiate benign from malignant lesions.35

Percutaneous needle biopsy or fine-needle aspiration cytology undertaken prior to referral is often misleading. Biopsy of these vascular tumours risks precipitating haemorrhage, and even an experienced histopathologist may experience difficulty in differentiating between liver cell adenoma and a well-differentiated hepatocellular carcinoma.

Management

In the symptomatic patient, surgical intervention will be required. A minority of patients will present with intraperitoneal bleeding, the cause of which might only be identified at laparotomy. Most deaths from liver cell adenomas are secondary to haemorrhage, with intraperitoneal bleeding carrying a 20% mortality rate in one series.22 Hepatic arterial embolisation36 or packing might be considered to facilitate transfer of the patient to a specialist centre. Definitive control of bleeding is best achieved by formal hepatic resection. In some patients, haemorrhage may be contained within the liver or subcapsularly. If the patient remains haemodynamically stable, it may be prudent to defer elective surgical intervention to enable resolution of the haematoma, thereby enabling a more limited hepatic resection (Fig. 4.6). Orthotopic liver transplantation has been described for unresectable benign liver tumours with severe symptoms and for patients with multiple adenomas.17,37

For the asymptomatic patient, surgical intervention should be considered; however, with new insights and understanding of the clinical–pathological and radiological features and recent innovations in molecular biology and immunohistochemistry, selected patients can now be managed conservatively.32,38 Steatotic liver cell adenomas have a minimal risk of bleeding or malignant transformation. Furthermore, several case reports document regression of liver cell tumours following cessation of oral contraceptives,39,40 although this is not a consistent finding, and development of hepatocellular carcinoma in the site of adenoma regression has been reported.32 Non-operative discrimination between liver cell adenoma and hepatocellular carcinoma remains challenging.

Focal nodular hyperplasia

Focal nodular hyperplasia (FNH) is a hyperplastic process in which all the normal constituents of the liver are present but in a disorganised pattern. The incidence of FNH has been increasing, although this is more likely to be related to improvements in abdominal imaging. Many lesions are still found incidentally at laparotomy or autopsy. About 90% of cases occur in women, primarily in the second and third decades, although the condition may also afflict older women and a small number of men and children. The incidence of FNH does not appear to have increased since the introduction of oral contraceptives; however, some investigators have suggested that oral contraceptives may foster growth or increased vascularity of these lesions, and they have been implicated in the few cases that present with haemorrhage.

Pathology

FNH consists of a firm lobulated localised lesion in an otherwise normal liver. These nodules are generally several centimetres in size and occasionally can grow much larger. Lesions are well circumscribed but have no capsule. On sectioning, there is generally a central scar with fibrous radiations which account for the nodular and sometimes umbilicated appearance. Lesions are usually similar or slightly lighter in colour than adjacent normal hepatic parenchyma (Fig. 4.7). FNH is multifocal in up to 20% of cases and may coexist with haemangiomas in 5–10% of patients.1

Microscopically, FNH looks similar to cirrhosis, with regenerating nodules and connective tissue septa. The lesions consist of many normal hepatic cells mixed with bile ducts or ductules and divided by fibrous septa. The septa contain numerous bile ducts and a moderate, predominantly lymphocytic, infiltration, and there is usually some evidence of mild cholestasis.

Clinical features

FNH is a benign process that rarely causes symptoms but the main difficulty lies in differentiating this process from other hepatic lesions. Less than 10% of patients with FNH have symptoms, the most common being mild, vague right upper quadrant abdominal pain. Acute symptoms due to haemorrhage are exceptional.

CT and MRI are important imaging modalities to characterise FNH. Classical CT appearance of FNH is of hyperattenuation during the arterial phase that becomes isoattenuating during the portal and delayed phase (Fig. 4.8). In approximately 40–60% of patients, the central scar will inially be hypoattenuating but becomes hyperintense in the delayed phase due to delayed washout of contrast. Typical MRI features of FNH are iso- or hypointensity on T1-weighted images, slight hyper- or isointensity on T2-weighted images and the presence of a central scar that appears hyperintense on T2-weighted imaging. After administration of gadolinium chelates, the appearance is similar to that seen on contrast-enhanced CT, i.e. dramatic enhancement in the arterial phase followed by isointensity during the portal venous phase with a high-intensity signal in the scar during the delayed phase. Cherqui et al.41 reported a 70% sensitivity and 98% specificity for MRI in detecting FNH.

Management

Treatment of a patient with FNH depends essentially on the certainty of the diagnosis. In asymptomatic patients with the typical features of FNH unequivocally demonstrated by one or more radiological investigation, no further treatment is required. However, a malignant tumour will be found in up to 6% of patients with an undetermined, presumed benign lesion.42 FNH on occasions may be difficult to differentiate from liver cell adenoma. If this is the case, it is advisable to proceed to biopsy of these lesions before committing to hepatic resection.

Data on the natural history of FNH have been gathered by Kerlin et al.43 Of 41 patients studied, 11 had lesions found incidentally at autopsy. Sixteen patients had open surgical biopsies of clinically apparent lesions, with the majority of the lesions left in situ. These patients were observed for up to 15 years, during which time none of the lesions bled or increased in size. The vast majority of patients with FNH can be managed conservatively, with surgical excision (enucleation or resection) only rarely considered for large symptomatic or complicated lesions.

Nodular regenerative hyperplasia (macroregenerative nodules)

This is a benign proliferative process in which the normal hepatic architecture is entirely replaced by diffuse regenerative nodules of hepatocytes. Autopsy reports suggest the prevalence of nodular regenerative hyperplasia (NRH) is approximately 2%. It predominantly occurs in older patients, and is often associated with lymphoproliferative and rheumatological diseases or develops after organ transplantation.

The majority of patients are asymptomatic, are diagnosed incidentally and require no further treatment. The most common physical findings are splenomegaly and hepatomegaly. A small percentage of patients may develop portal hypertension due to compression of intrahepatic portal radicles by the regenerating nodules, and present with variceal bleeding or ascites. Rarely, patients may develop hepatic failure and in some instances have undergone liver transplantation. Liver function tests are usually normal or slightly elevated, and the radiological features are relatively non-specific. The diagnosis of NRH is confirmed on the gross and histological findings of the liver. Macroscopically, the hepatic parenchyma is entirely replaced by nodules varying in size from 0.1 to 4 cm. The histological findings of NRH are regenerating hepatocytes separated by atrophic parenchyma, curvilinear compression of the central lobule and absence of fibrous tissue or bands of scar tissue between the nodules. NRH may be suspected when a patient presents with symptoms of portal hypertension and a liver biopsy that fails to show cirrhosis or is interpreted as being normal. Confirmation may require targeted liver biopsy. Liver cell dysplasia is a common finding in NRH and there are a small number of case reports of hepatocellular carcinoma developing in livers with NRH, leading some authors to suggest that NRH may represent a premalignant condition in some patients.

Bile duct adenoma (bile duct hamartoma)

Surgeons should be aware of bile duct adenomas since they are common and may be mistaken at operation as liver metastases. They do not manifest clinically but are incidental findings at laparotomy or autopsy.44 They rarely exceed 1 cm in diameter and appear as raised greyish-white areas on the liver capsule. Histologically, they are composed of a mass of mature bile ducts surrounded by fibrous stroma, which blends indistinctly into the adjacent liver. They require to be distinguished from the nests of hyperplastic bile ducts that occur in focal nodular hyperplasia and also in undifferentiated adenocarcinoma of the biliary tract type.

The only clinical significance of bile duct adenoma is its possible confusion at laparoscopy or laparotomy with metastatic carcinoma, cholangiocarcinoma or other focal hepatic lesions. When encountered, excisional biopsy should be performed to confirm the diagnosis.

Hepatic pseudotumours

Hepatic pseudotumours may be considerable in size and can occur in any age group. These lesions are essentially overgrowths of chronic inflammatory tissue but may be mistaken for other neoplastic lesions of the liver.45 The aetiology is not known but they may be secondary to thrombosis and infarction of a major vessel, represent a form of immune reaction, or result from resolution of an abscess. They may be either hyperechoic or hypoechoic on US and appear as a hypodense lesion on CT. Such pseudotumours may require resection to prevent reactivation of infection. The clinical history and presentation are likely to point towards a diagnosis of pseudotumour.

Miscellaneous benign tumours

Mesenchymal hamartomas are exceptional and probably of congenital origin. They are most commonly described in infants under 12 months; however, a few have been documented in adults.46 Although they are entirely benign, hamartomas can compromise the liver and the individual by progressive enlargement, and therefore these lesions should be resected. Microscopically, the tumour is characterised by a myxoid background of highly cellular embryonal mesenchyme, throughout which are found random groups of hepatic cells, bile ducts and multiple cysts, which may produce a honeycomb appearance. Recurrence following excision has not been reported.

Primary myxoma in the adult is exceptional. Primary lipomas are rarely described in life but have been identified incidentally at post-mortem.1 Other solid tumours include leiomyoma, mesothelioma and fibroma. Benign teratoma of the liver has been reported but this generally occurs in children.

Liver abscess

The incidence of pyogenic liver abscess has remained relatively constant over the past century despite earlier diagnosis and treatment of underlying causes and more aggressive antibiotic therapies. In recent years, the decrease in cases resulting from haematogenous spread from infected foci has been mirrored by an increase in cases secondary to hepatobiliary pathology. In almost half the patients reviewed over a 5-year period, biliary sepsis was the major predisposing factor.47 In 20% of patients, the presumed source of infection was from the portal route, but few cases were thought to have arisen from systemic infection. Hepatic abscesses secondary to ascending cholangitis are often multiple due to the distribution of the infecting organism along the biliary ductal system.48 Early reports implicated choledocholithiasis as the main causative factor; however, more recent series document malignant biliary obstruction as a more common aetiological factor.47,49

Infections within organs drained by the portal vein are dependent on the underlying illness. In the early literature, portal vein pyelophlebitis secondary to appendicitis was often implicated, whereas diverticulitis, pancreatitis and diffuse peritonitis are now more frequently reported. Haematogenous spread from non-gastrointestinal sources accounts for 10–20% of liver abscesses and occurs most typically with bacterial endocarditis, other conditions associated with systemic bacteraemia such as urinary sepsis, pneumonia and osteomyelitis or following intravenous drug abuse. Abscesses may also occur from direct extension into the liver parenchyma from localised perforation of an adjacent viscus, such as the gallbladder, colon, stomach or duodenum. In a significant percentage of patients (approximately 15–35%), the aetiology of hepatic abscess remains obscure (cryptogenic abscess) despite extensive clinical and pathological investigation.

Clinical presentation

Patients present with a spectrum of symptoms and signs, the most consistent being fever associated with malaise, anorexia, weight loss and upper abdominal pain. Jaundice is a feature in approximately 50% of cases. Laboratory studies typically reflect a systemic bacterial infection. Commonly reported findings are of leucocytosis, anaemia, hyperbilirubinaemia, hypoalbuminaemia and raised levels of acute-phase proteins. US is invariably diagnostic and will often demonstrate a fluid-filled cavity. There may be a hyperechoic wall, the presence of which is dependent on the chronicity of the abscess. CT may be useful to exclude the presence of other abscesses and to identify a primary source within the abdomen (Fig. 4.9). Magnetic resonance cholangiography should be undertaken in patients with biliary symptoms, obstructive liver function tests or a dilated common bile duct, and can be combined with cross-sectional MRI to identify any hepatic parenchymal abnormality. Barium enema or colonoscopy may be indicated to exclude a colonic source of portal pyaemia.

Management

The key to successful management is drainage of the purulent collection combined with appropriate antibiotic therapy, which is determined by the results of culture of blood and aspirated pus. Although virtually all pathogenic organisms have been identified, enteric organisms predominate. Polymicrobial infection is seen frequently when hepatic abscess is secondary to infection arising from the portal venous system. Although antibiotic therapy as the sole treatment for hepatic abscess is rarely successful, prolonged systemic antibiotic administration may be the only option for patients with diffuse multiple microabscesses. In general, macroscopic hepatic collections require drainage of the purulent material. Over the past two decades, the introduction and refinement of percutaneous drainage techniques have dramatically altered the management of patients with pyogenic hepatic abscesses. Percutaneous drainage has become the first-line therapeutic option in most centres for patients with single or multiple liver abscesses.48,50,51 Abscess communication with the intrahepatic biliary tree does not prevent pyogenic collections being successfully treated by percutaneous techniques, although the period of drainage may be prolonged. The use of percutaneous aspiration combined with systemic antibiotics without drainage has been advocated by some groups;52 however, in the only randomised trial comparing the two techniques, aspiration was successful in only 60% of patients whereas percutaneous catheter drainage was successful in 100% of patients.53

Regular irrigation of drainage catheters reduces the risk of catheter blockage due to necrotic debris. Surgical drainage is rarely employed but is usually reserved for patients who have failed percutaneous drainage and those who require surgical management of the underlying problem. Liver resection is occasionally required for patients with liver abscess.54 The indication is usually failed non-operative management, hepatolithiasis, intrahepatic biliary stricture or gross parenchymal destruction.

Effective decompression of the biliary tree is as important as abscess drainage where obstruction of the bile duct has contributed to the development of hepatic abscess. Following successful drainage of the abscess, antibiotic administration should be continued for a prolonged period (4–6 weeks) to assist in the complete eradication of infection.

Pyogenic liver abscess still carries a significant mortality. A significant number of patients will not survive admission to hospital, reflecting the high proportion of patients developing hepatic abscess related to underlying malignant biliary obstruction.

Amoebic abscess

This form of abscess is sufficiently common that it should be considered in the differential diagnosis of hepatic lesions. About 10% of the world’s population is chronically infected with Entamoeba histolytica, although less than 10% of individuals are symptomatic. Liver abscess is the most common extraintestinal manifestation of amoebiasis and is reported in 3–10% of affected patients. Males are more commonly affected than females, and the highest incidence is in the 20- to 50-year-old age group.55

The diagnosis is likely to be straightforward in areas where amoebiasis is endemic but the liver abscess may present many years after previous intestinal infection. Some 75–90% of abscesses are in the right lobe, and involvement of the left lobe usually indicates more advanced disease. Rupture occurs in 2–17% of cases and usually occurs into the peritoneal cavity and rarely into the pleural cavity, the bronchial tree or pericardium. Signs and symptoms of amoebic infection are the same as for pyogenic abscess. On US and CT, the boundaries of the abscess are generally poorly defined (Fig. 4.10). Patients with amoebic liver abscess virtually always have serum antiamoebic antibodies, which can be detected by an indirect haemagglutination test or an enzyme-linked immunosorbent assay (ELISA) technique. Percutaneous aspiration produces a sterile and odourless fluid, which is described as having the appearance of ‘anchovy paste’. Routine percutaneous aspiration is now regarded as superfluous in the management of amoebic liver abscess unless serology is inconclusive, a therapeutic trial with antiamoebic drugs is deemed inappropriate (as in pregnancy), or rupture is suspected to be imminent. A preliminary diagnosis can be made on the basis of a dramatic clinical response to metronidazole, which should be commenced empirically in endemic areas.55 If clinical symptoms do not resolve within 48–72 hours of treatment, an incorrect diagnosis or secondary bacterial infection should be suspected. Percutaneous aspiration may be beneficial for patients when medical treatment has failed. Percutaneous catheter drainage is indicated rarely as the abscess contents are viscous and bacterial superinfection may occur. Open surgical drainage is indicated in complicated cases and in those who fail to respond to conservative therapy. In a meta-analysis of 3081 patients with amoebic liver abscess the mortality rate was 4%, compared with a mortality rate of 46% in patients with pyogenic liver abscess.56

Hydatid cyst

Echinococcus infection is a zoonosis that can give rise to liver lesions. These collections are better classified as cysts rather than abscesses because the organism is almost entirely determined by the hepatic environment and little host inflammatory reaction is present. An intense fibrous reaction around the lesion is characteristic but there is no epithelial lining to the cyst. The incidence of Echinococcus granulosus is in decline but sporadic cases are reported in Europe, Australia, New Zealand, South America, Asia and Africa. The prevalence of human echinococciasis is directly related to contact with dogs and sheep. Echinococcus multilocularis, or alveolar hydatid disease, is rare, although it is a much more dangerous condition. It pursues a more invasive course than the more common form of the disease.

Hydatid cysts are most commonly unilocular and may grow as large as 20 cm. The cyst wall is about 5 mm thick and consists of an external laminated hilar membrane (ectocyst layer) and an internal enucleated germinal layer (endocyst layer), which is responsible for production of the colourless hydatid fluid, brood capsules and daughter cysts. Brood capsules are small cellular masses and together with calcareous bodies form ‘hydatid sand’. About 70% of lesions are in the right lobe and 15% in the left, with both lobes involved in approximately 15% of cases.

Clinical presentation

Many infections are probably contracted during childhood and lie latent for many years, often until complications occur. Clinical symptoms of echinococcal cystic disease are often insidious but there is usually a history of contact with dogs or sheep. Distension of the liver capsule may produce right upper quadrant pain. Jaundice is infrequent but may be due to extrinsic biliary compression or due to rupture into the biliary tree leading to obstruction by cystic debris. Secondary bacterial infection of the cyst occurs in approximately 10% of cases. Liver function tests are generally abnormal and eosinophilia is present in up to one-third of patients.

Echinococcal disease may occasionally mimic a primary liver tumour or metastatic disease. Serology may be helpful in establishing a diagnosis. Plain abdominal radiographs may reveal a calcified cyst wall. US and CT may demonstrate septa, ‘hydatid sand’ or daughter cysts within the main cyst cavity, which are important signs for differentiating hydatid from other benign liver cysts (Fig. 4.11). Percutaneous aspiration and drainage should be avoided because of the risk of dissemination or anaphylaxis.

Management

Once the diagnosis has been established, surgery is generally required, as the natural history of viable hydatid cysts is one of growth and potential complications. Significant morbidity and mortality may result from rupture into the peritoneal or thoracic cavity or the development of a bronchobiliary fistula. Surgery might best be avoided in elderly frail patients with small, asymptomatic calcified cysts. Treatment with an oral anthelmintic agent, such as mebendazole or albendazole, to minimise the risks of hydatid spread at surgery or reduce the incidence of postoperative recurrence, has been advocated by some authors, although there remains considerable doubt as to its efficacy. Aspiration of the hydatid cyst with instillation of scolicidal agents, such as hypertonic saline, silver nitrate, chlorhexidine, cetrimide, hydrogen peroxide, formalin or alcohol, has generally been abandoned because of the risk of anaphylaxis or the risk of developing sclerosing cholangitis if a bile duct communication is present. These have been generally replaced by perioperative cover with an anthelmintic agent.

The main principle of surgical treatment is to eradicate the parasite, prevent intraoperative spillage of cyst contents and obliterate the residual cavity.57 At open operation, the operating field is generally packed off with swabs. After decompression, the cyst and contents are shelled out by peeling the endocyst off the host ectocyst layer. The fibrous host wall of the residual cavity should be carefully examined for any bile leakage from biliary–cyst communications, which are then sutured. The residual cyst cavity can be marsupialised, packed with omentum or plicated.58 Pericystectomy is advocated by some but should preserve those portions of the cyst wall that come into contact with major blood vessels. For smaller, peripheral lesions, formal hepatic resection may be considered, particularly if a diagnostic dilemma remains. The mortality for surgery of hydatid disease should be low and confined to complicated disease. In a series of 505 patients, Milicevic reported a mortality rate of 1.5% and a morbidity rate of 30%.58

Simple cysts of the liver

Non-parasitic cystic disease of the liver can result from a congenital malformation of the intrahepatic bile ducts. These cysts may be single, multiple or diffuse (polycystic liver disease). They contain serous fluid and do not communicate with the intrahepatic biliary tree. Small cysts are surrounded by normal liver tissue, although as these enlarge there is displacement and atrophy of adjacent hepatic tissue. A large cyst may occupy an entire lobe of the liver and result in compensatory hypertrophy of the residual liver. Such cysts have no vascularised septa and are unilocular. Microscopically, they are lined by a single layer of cuboidal or columnar epithelial cells, which resemble those of biliary epithelium. Simple cysts have a prevalence of about 3.6%. The female to male ratio is 4:1 in asymptomatic cases, but rises to 10:1 in symptomatic or complicated simple cysts.59

Clinical presentation

The vast majority of simple cysts are asymptomatic and are discovered incidentally. Large cysts may cause abdominal pain or discomfort, and a mass may be palpable in the right hypochondrium. Other symptoms may include anorexia, early satiety or vomiting. Rare complications include acute onset of pain from intracystic haemorrhage, rupture, torsion or infection. Jaundice is uncommon, but may be caused by external compression of the biliary tree. Likewise, portal hypertension has been reported as a consequence of portal vein compression.

Diagnosis can be made on the basis of abdominal US, which demonstrates a circular anechoic area that has a well-defined boundary with the liver. No wall is evident and there is posterior acoustic enhancement. Intracystic haemorrhage may cause internal acoustic shadowing; however, the presence of cyst wall nodules or solid intracystic components must be considered neoplastic. US examination of the kidneys is useful in patients with multiple liver cysts to exclude the presence of polycystic disease. Further diagnostic investigation is rarely required, although where intervention is contemplated, CT or MRI will provide more accurate anatomical localisation and exclude the presence of other cysts. Cysts appear as well-rounded, water-dense lesions without septa on CT (Fig. 4.12). Intravenous contrast enhancement will confirm the avascularity of these lesions. Where complications such as haemorrhage occur, the simple cyst may appear relatively thick-walled and may contain cystic debris. In such instances, serological tests should be undertaken to exclude parasitic infection. It should be borne in mind that calcification is rarely present in simple cysts but may be present with hydatid cysts.

Management

Asymptomatic simple cysts require no treatment; however, symptomatic or complicated simple cysts may require intervention. Percutaneous aspiration risks introducing infection and does not provide definitive therapy; however, this technique may be useful as a diagnostic test for patients with questionable symptoms.60 Aspiration followed by percutaneous instillation of sclerosant agents has shown promising results in reducing symptomatic and radiological cyst recurrence.61 Open deroofing of simple liver cysts has, in the past, been the established conventional treatment. Total cystectomy is not required and may be hazardous since there is no plane of dissection between the cyst and the liver. In recent years, laparoscopic deroofing of such solitary cysts has been advocated. This technique was first described in 1991,62 and is associated with higher patient acceptability and shorter postoperative stay compared with open surgical techniques. In a recent comprehensive review of 21 papers on the laparoscopic management of hepatic cysts, Klingler et al.63 reported 61 laparoscopic deroofing procedures with an overall morbidity rate of 10%.

Even at open surgery, deroofing of large centrally placed cysts may not prevent reconstitution of the cyst with recurrence of symptoms. In such patients, more radical resection that does not generally involve substantial sacrifice of functioning hepatic parenchyma should be considered.

Polycystic liver disease (PCLD)

Adult polycystic kidney disease is frequently associated with multiple liver cysts, which are macroscopically and microscopically similar to simple cysts of the liver. However, in this condition the liver cysts are multiple when present and may extensively replace both lobes of the liver (Fig. 4.13). In addition to the macroscopic cysts, there are usually numerous microscopic cysts and clusters of multiple bile ductules, designated as von Meyenburg complexes. The condition is an autosomal dominant disorder and carries a much more sinister prognosis because of the risk of chronic renal failure. There is an increased prevalence associated with increasing age and the female sex.64

Clinical presentation

In most patients with adult polycystic kidney disease, the polycystic hepatomegaly is clinically silent. The commonest symptoms are related to increase in liver size, and include abdominal and pelvic discomfort and respiratory compromise. An abdominal mass will be present in three-quarters of patients. There are rarely signs of cholestasis, liver failure or portal hypertension, and liver function tests are usually normal. Both US and CT will demonstrate multiple fluid-filled cysts with well-defined margins in the liver and the kidneys (Fig. 4.14). Liver cysts increase in size slowly and complications are uncommon. Rupture and bacterial infection are reported to be more common with immunosuppression following kidney transplantation.65

Management

Asymptomatic patients require no treatment. Percutaneous aspiration of cysts and instillation of sclerosant rarely produce satisfactory long-term relief of symptoms. Surgical deroofing or fenestration according to the technique described by Lin et al.66 is the most widely used treatment modality for symptomatic patients but must be extensive and radical to achieve satisfactory results. Some have suggested that laparoscopic deroofing may provide good relief of symptoms.67 However, in our own series this technique was associated with a high recurrence rate.59 Recent evidence suggests that a more aggressive open surgical approach involving resection of the liver may provide longer-lasting relief of symptoms,68,69 but it should be appreciated that hepatic resection is difficult in such patients and is associated with significant morbidity. Nonetheless, extensive resection and cyst deroofing may allow the abdomen to better accommodate the enlarged residual liver. Surgical intervention is often associated with transient but massive ascites in the postoperative period.70 Liver transplantation may be indicated in selected patients with hepatic failure.71

Cystadenoma

Cystadenoma of the liver is rare, but it has a strong tendency to recur and has a malignant potential. It is usually solitary and mainly affects women over 40 years of age. Cystadenomas are often multiloculated and may measure up to 20 cm in diameter. Histologically, the locules are mostly lined by a single layer of cuboidal or columnar cells; however, in areas the epithelium may form papillary projections. The presenting features are similar to other mass-forming hepatic pathologies, namely abdominal discomfort, anorexia, nausea and abdominal swelling. A large hepatic mass may be palpable. Liver function tests are usually normal. Diagnosis is based on US, MRI or CT (Fig. 4.15). US characteristics are of a large, anechoic, fluid-filled area with irregular margins. Internal echoes may be seen due to septa or papillary projections from the cyst wall. CT provides more accurate localisation, but may be less sensitive than US for demonstrating the thin septations. Cystadenomas grow very slowly and complications include biliary obstruction, intracystic haemorrhage, bacterial infection, rupture, recurrence after partial excision and transformation into cystadenocarcinoma. This may be suspected radiologically by the presence of large projections into the cyst lobules and septal calcification.72 Cystadenoma of the liver, even if asymptomatic, must be treated by complete excision.

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